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Start Over Author "Zuccheri G" Journal chemphyschem
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3. Protein Unfolding and Refolding Under Force: Methodologies for Nanomechanics

Author

P. Baschieri, Giampaolo Zuccheri, Bruno Samorì, Samorì B., Zuccheri G., and Baschieri P.

Subjects
Protein Denaturation, Protein Folding, Protein Conformation, Biophysics, Muscle Proteins, Myosins, Microscopy, Atomic Force, Protein Structure, Secondary, Protein structure, Microscopy, Animals, Nanotechnology, Connectin, Physical and Theoretical Chemistry, Chemistry, Physical, Atomic force microscopy, Chemistry, Proteins, ATOMIC FORCE MICROSCOPY, DNA, OPTICAL TWEEZERS, Atomic and Molecular Physics, and Optics, Optical tweezers, Intramolecular force, PROTEIN UNFOLDING, Protein folding, Stress, Mechanical, Protein Kinases, Nanomechanics
Abstract

An increasing number of inter- and intramolecular interactions can nowadays be probed using single-molecule manipulation techniques. Protein unfolding and refolding is the most representative--though complex--of these interactions. Herein, we review the main modes of performing a force unfolding experiment: the velocity clamp and the new force clamp mode. We also compare some of the physical aspects behind the two most frequently used single-molecule manipulation instrumentations: optical tweezers and atomic force microscopes.

Published
2004
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