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OBJECTIVE To study the chemical constituents in roots of Peucedanum praeruptorum from Henan. METHOD The constituents were isolated by column chromatography on silica gel and ODS, and identified by NMR, MS spectroscopic methods. RESULT Six compounds, Pd-I b(1), marmesinsin (2), rutarin (3), isorutarin (4), 4H-1-benzopyran-4-one, 5-hydroxy-6-methoxy-2-phenyl-7-O-alpha-D-glucuronyl methyl ester (5), 4H-1-benzopyran-4-one, 5-hydroxy-6-methoxy-2-phenyl-7-O-alpha-D-glucuronyl acid (6) were isolated and identified. CONCLUSION Compound 5 was a new compound, compounds 5 and 6 were isolated from Peucedanum plant for the first time.

Objective To compare the components and contents of volatile oil in Gardenia jasminoids, Zingiber officinale, G. jasminoids roasted with ginger juice and to provide scientific basis for intrinsic material of G. jasminoids roasted with ginger juice. Method The volatile oil in the three herbal medicines was extracted by steam distillation, the components were analyzed by GC-MS and the contents of the components were calculated by area normalization method. Result The contents of volatile oil in G. jasminoids, Z. officinale, G. jasminoids roasted with ginger juice were 1.0, 2.0, 1.5 microL x g(-1), respectively. A total of 89 components were identified by GC-MS. Although 42, 75 and 77 peaks were separated, only 30, 58 and 67 components were identified accordingly, which accounted 94.1%, 90.52% and 94.38% of the whole volatile oil. Conclusion After being processed with ginger juice, the components and contents of volatile oil in G. jasminoids has been changed obviously.

OBJECTIVE To establish the HPLC fingerprint method and compare the changes of chemical compositions of the processed products from Sinapis alba. METHOD The procedure of HPLC analysis was performed on a agilent TC-C18 (2) column at 35 degrees C with the acetonitrile -0.1% phosphoric acid in gradient elution as the mobile phase. The detection wavelength was set at 254 nm, and the flow rate was 1.0 mL x min(-1). The similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine (Version 2004 A) was applied to analyze the similarity. RESULT The average similarities among the processed products were over 0.96, the standard HPLC fingerprint and five main chromatographic peaks with the isolated compounds was obtained and identified. CONCLUSION The established methods with two solvents are suitable for the HPLC fingerprints determination which elementary elucidate the scientific intensions of breaking the enzyme for glycosides.

OBJECTIVE To compare the contents of two another anthraquinone-O-glucosides in five different processed products from Rheum palmatum. METHOD Aloe-emodin-3-CH2-O-beta-D-glucopyranoside and emodin-8-O-beta-D-glucopyranoside were determined simultaneously by HPLC on an Agilent TC-C18 (2) column at 35 degrees C with the tetrahydrofuran-1% glacial acetic acid (25:75). The detection wavelength was set at 410 nm and the flow rate was 0.9 mL x min(-1). RESULT The calibration curves for two components were linear within the range of 0.0090-0.2704 microg (r = 0.9996), 0.0396-1.188 microg (r = 0.9996), and the average recoveries were 97.85%, 97.19% respectively. CONCLUSION The results showed the remarkable variation regulations among the five different processed pieces. The contents of the crude pieces and the pieces roasted vinegar were almost the same, while the pieces roasted with millet wine were a little higher than that of the crude pieces, and the contents at the two components in prepared pieces and charring pieces evidently reduced.

OBJECTIVE To study the inhibitory effect of medicated serum of SLW on estrogen production and to approach on the key mechanism of SLW in inhibiting the invasion ability of endometrial cells of endometriosis. METHOD First, the model of eutopic primary cultured endometrial cells of endometriosis and hysteromyoma in vitro was successfully established. Taking that of endometrial cells of hysteromyoma as control, the secretion level of E2 of endometrial cells in the culture media supernatant at different time point with the treatment of high, middle and low dose of SLW serum was detected by electrochemiluminescence immunoassay, the activities of matrix metalloproteinase-2, 9 (MMP-2, 9) were detected by gelatinase zymography assay, and the expression of tissue inhibitor of metalloproteinase-1, 2 (TIMP-1, 2) protein was observed by immunofluorescence. After the optimal time for SLW to inhibit invasion ability of endometrial cells was identified based on time-effect relationship, another endometrial cells were divided into six groups: hysteromyoma endometrium group, eutopic endometrium of endometriosis group, eutopic endometrium of endometriosis + middle dose of SLW serum group, eutopic endometrium of endometriosis + middle dose of SLW serum + E2 group, eutopic endometrium of endometriosis + anastrozole serum group , and eutopic endometrium of endometriosis + E2 group. The activities of MMP-2, 9 and the expression of TIMP-1, 2 protein were detected according to the optimal time point. RESULT The secretion level of E2 of eutopic endometrium in endometriosis could be decreased by SLW, which showed the dependence of time and concentration. The result of gelatinase zymography assay and immunofluorescence respectively showed that along with the time the activities of MMP-2, 9 of eutopic endometrial cells of endometriosis were significantly higher than those of hysteromyoma at the same time point (P < 0.01). After 48 hours, with the treatment of middle dose of SLW serum, the activities of MMP-2, 9 of eutopic endometrial cells of endometriosis could be decreased (P < 0.01) while the expression of TIMP-1, 2 protein could be increased obviously (P < 0.01). The malignant invasion ability improved by SLW of eutopic endometrial cells of endometriosis was partly recruited by add-back E2 treatment. There was no significant difference in the activity of MMP-2 and the expression of TIMP-1, 2 protein between eutopic endometrium of endometriosis + middle dose of SLW serum + E2group and untreated group. The behavior of invasion of endometrial cells of endometriosis could be deteriorated treated by E2 as contrast to anastrozole, a specific aromatase inhibitor. CONCLUSION SLW could decrease the secretion of E2 so as to inhibit the invasion of the eutopic endometrial cells of endometriosis.

Objective To compare of the contents of two stilbene glycoside in five processed products of Rheum palamatum. Method The contents of trans-3, 5, 4'-trihydroxystilbene-4'-O-beta-D-glucopyranoside (a) and trans-3, 5, 4'-trihydroxystilbene-4'-Obeta-D-(6"-O-galloyl)-glucopyranoside (b) were determined by HPLC analysis at 35 degrees C with methanol-1% acetic acid as mobile phase, the wavelengths were set at 280, 300 nm, the flow rate was 1.0 mL x min(-1). Result The two components could be detected in five processed products, and their contents were more close in the no-parched pieces, the vinegar roasts pieces and the wine roast pieces. However, the contents reduced significantly in other two kinds of pieces which were lower than 80 percent of no-parched pieces. Conclusion High temperature may result in a significant reduction on glycoside in the pieces of rhubarb, and we have received similar results from determination of other glycoside compounds. Further analysis and comparison with the content of their corresponding aglycones, can provide a scientific basis to explain the variation of the material basis in the processed products of rhubarb.