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Objective To investigate the contamination status of Vibrio cholerae in foods of amphibians and reptiles during circulation and cultivation in Guangzhou in 2019, and we provide evidence for the food safety supervision and further prevention and control of food-borne cholerae outbreaks. Methods According to population and regional distribution, characteristics of food production, circulation and consumption features of Guangzhou, amphibian and reptile foods (live turtles and frogs) were collected from supermarkets, farmer s market, wholesale market in five districts of Guangzhou and cultured areas of amphibians and reptiles in Shantou city by random sampling in March, May, August, October and November of 2019, and Vibrio cholerae was detected in these samples in line with Manual of Vibrio cholerae Prevention and Control. Results A total of 127 live turtles and frogs were collected, and the prevalence of Vibrio cholerae was 4.72%(6/127). The prevalence of Vibrio cholerae in frog samples was 6.90%(6/87), while O1 and O139 Vibrio cholerae was found negative for all turtle samples. Vibrio cholerae was detected in samples from Baiyun District, Liwan District and Shantou city. The cholerae-positive samples were collected from farmer s market, wholesale market and farm; From the sample area, 6 positive samples were mainly derived from other cities in Guangdong Province and outside the Province; According to the sampling time, Vibrio cholerae was detected only in October and November. The distribution of Vibrio cholerae positive samples in different regions, places, places of origin and months was not statistically significant (P>0.05). All isolated strains were categorized into Inaba of O1 group, and the virulence gene ctxA was negative. Conclusion The contamination level of Vibrio cholerae was relatively low in amphibian and reptile foods of Guangzhou, more attention should be paid to the cholera contamination in frogs during breeding period. [ABSTRACT FROM AUTHOR]