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5 results on '"Zhengwang Zhang"'

2. Emei Shan Liocichla: population, behavior and conservation

Author

Yiqiang Fu, Simon D. Dowell, and Zhengwang Zhang

Subjects
Emei shan liocichla, education.field_of_study, Geography, biology, Population, General Engineering, Zoology, biology.organism_classification, education
Published
2013

5. Improvement on molecular sex identification primers for Passeriform bird species

Author

Ying-Ying Liu, Zhengwang Zhang, Jianqiang Li, and Ning Wang

Subjects
Genetics, Passeriforme, Lonchura striata, biology, Evolutionary biology, General Engineering, Identification (biology), Limiting, Aegithalos concinnus, Primer (molecular biology), biology.organism_classification, Sex allocation
Abstract

The primer pair sex1/sex2, which can be widely applied for sex identification in Galliform species, was used to determine the sex of 17 Passeriform species. As CHD-W fragments tended to be preferentially amplified, which may cause unnecessary misidentification in bird species with little difference between CHD-Z and CHD-W, we modified sex1 and sex2, obtaining sex1' and sex-mix respectively. Primer sets were then recombined to conduct sex identification. After testing several Passeriforme birds of known sex, we found that the primer pair sex1'/sex2 was better at limiting the preferential amplification of CHD-W fragments. As they are being frequently used in sex allocation study of Aegithalos concinnus and song learning research of Lonchura striata, we can expect more applications of this primer pair to further studies in Passeriformes.

Published
2010

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