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Objective To explore the effects of Semen Cuscutae-Fructus Lycii（SC-FL） on testicular structure and sperm quality of oligoasthenozoospermia mouse models induced by Glucosides of Tripterygium wilfordii hook. F（GTW）. Methods A total of 36 male BALB/c mice were randomly divided into the control group（12） and the model group（24）. The mice in the control group were treated with deionized water, and the mice in the model group with GTW for 4 weeks. Two mice from the control group and four mice from the model group were randomly selected for model verification. After the model was successfully validated, the remaining 10 normal mice were used as the control group, and the mice in the model group were divided into the model group and the treatment group according to the random number table method, with 10 mice in each group. The mice in the control group and the model group were given with deionized water, and the mice in the treatment group intragastrically with SC-FL suspension for 4 weeks. At the end of the 8th week of the experiment, the body mass, testicular mass and semen parameters of mice were measured, and the histological morphology of testes was observed by hematoxylin eosin（HE） staining. Results The testicular quality, sperm concentration, and total sperm motility（PR+NP） of mice in the treatment group were higher than those in the spermatogenic failure model group, with significant differences（P＜ 0.01）. Compared with the model group, the Sertoli cells and spermatids were increased in the testicular tissue of the treatment group, and the seminiferous tubules were structurally intact and arranged closely. Conclusion Semen Cuscutae-Fructus Lycii（SC-FL）can effectively alleviate oligoasthenozoospermia, and its mechanism may be related to the repair of testicular tissue structure damage. [ABSTRACT FROM AUTHOR]

Objective To establish a simple and efficient method for isolation and culture of mouse spermatogonial stem cell（mSSC） in vitro. Method（s） The cell suspension from testis of male fetal Kunming mice and C57BL/6mice was obtained by mechanical separation and enzyme digestion. The cell suspension containing mSSC cells and Sertoli cells and other cells was co-cultured until the clone of mSSC formed. When Sertoli cells were not in period of proliferation, the clones of mSSC were removed to the feeding layer cells. The isolated mSSC cells were further identified by molecular markers and induced differentiation. Result（s） The mSSC cells isolated by this co-culturing method from different genetic backgrounds of male fetal Kunming and C57BL/6 mice could be cultured for several generations in vitro. The result of RT-PCR showed that specific molecular markers, such as Plzf, Gfrα-1 and Ngn3 were expressed in mSSC cells. mSSC cells treated with retinoic acid presented the phenotype of sperm-like cells. The amount of haploid in induced mSSC cells accounted for 9.52%, while no haploid in the control mSSC cells. The induced mSSC expressed differentiation-associated genes such as Acrosin, Th2b and Sycp3. Conclusion（s） In this study, we developed the co-culturing method which could isolate and culture mSSC cells from different genetic background mice. This method not only simplifies the separation steps, but also saves the cost of culture, which will provide basis for the study of mSSC cells. [ABSTRACT FROM AUTHOR]