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t:The present study investigated the influence of taurochenodeoxycholic acid (TCDCA) on EP2 receptors on synovial fibroblast (FLS) from adjuvant arthritis (AA) rat. The FLS from AA rat were cultured in vitro and used Elisa method to measure the quantity of second messenger cAMP, in order to determine the best drug concentration of TCDCA. And then, with the treatment of TCDCA in vitro, using real time RT-PCR measured EP2 receptors expression on FLS. The resule of Elisa method indicated that after TCDCA treatment, the concentration of cAMP had asignificantly change in FLS when existence of PGE2 (10–6mol/L), with dose-dependent. In addition, real-time RT-PCR results showed that PGE2 could promote EP2 mRNA expression in the FLS, while TCDCA(10–5-10–7mol/L) could significantly reduce the mRNA expression of EP2 receptors (P < 0.01). These results indicated that TCDCA may have an impact on PGE2 pathway, which may be one of a new breakthrough point for further research TCDCA biological effects. [ABSTRACT FROM AUTHOR]

The article presents a study which examines the anti-inflammatory action of taurochenodeoxycholic acid (TCDCA). Researchers perform an intragastric administration of TCDCA into adjuvant arthritis rats, and measure the contents of nitric oxide (NO) and lymphotoxin-beta (LTB4) of peripheral blood by fluorospectrophotometry and enzyme-linked immunosorbent assay (ELISA). Results show the relationship between the anti-inflammatory action of TCDCA and production of LTB4 and NO .