43 results on '"Cheng, Liu"'
Search Results
2. Head-to-head comparison of the test performance of self-administered qualitative vs. laboratory-based quantitative fecal immunochemical tests in detecting colorectal neoplasm
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Ming Lu, Yu-Han Zhang, Bin Lu, Jie Cai, Cheng-Cheng Liu, Hong-Da Chen, Min Dai, and Jing Ni
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Medicine - Abstract
Abstract. Background:. Fecal immunochemical tests (FITs) are the most widely used non-invasive tests in colorectal cancer (CRC) screening. However, evidence about the direct comparison of the test performance of the self-administered qualitative a laboratory-based quantitative FITs in a CRC screening setting is sparse. Methods:. Based on a CRC screening trial (TARGET-C), we included 3144 pre-colonoscopy fecal samples, including 24 CRCs, 230 advanced adenomas, 622 non-advanced adenomas, and 2268 participants without significant findings at colonoscopy. Three self-administered qualitative FITs (Pupu tube) with positivity thresholds of 8.0, 14.4, or 20.8 μg hemoglobin (Hb)/g preset by the manufacturer and one laboratory-based quantitative FIT (OC-Sensor) with a positivity threshold of 20 μg Hb/g recommended by the manufacturer were tested by trained staff in the central laboratory. The diagnostic performance of the FITs for detecting colorectal neoplasms was compared in the different scenarios using the preset and adjusted thresholds (for the quantitative FIT). Results:. At the thresholds preset by the manufacturers, apart from the qualitative FIT-3, significantly higher sensitivities for detecting advanced adenoma were observed for the qualitative FIT-1 (33.9% [95% CI: 28.7–39.4%]) and qualitative FIT-2 (22.2% [95% CI: 17.7–27.2%]) compared to the quantitative FIT (11.7% [95% CI: 8.4–15.8%]), while at a cost of significantly lower specificities. However, such difference was not observed for detecting CRC. For scenarios of adjusting the positivity thresholds of the quantitative FIT to yield comparable specificity or comparable positivity rate to the three qualitative FITs accordingly, there were no significant differences in terms of sensitivity, specificity, positive/negative predictive values and positive/negative likelihood ratios for detecting CRC or advanced adenoma between the two types of FITs, which was further evidenced in ROC analysis. Conclusions:. Although the self-administered qualitative and the laboratory-based quantitative FITs had varied test performance at the positivity thresholds preset by the manufacturer, such heterogeneity could be overcome by adjusting thresholds to yield comparable specificities or positivity rates. Future CRC screening programs should select appropriate types of FITs and define the thresholds based on the targeted specificities and manageable positivity rates.
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- 2021
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3. Clinical profiles and outcomes of acute type A aortic dissection and intramural hematoma in the current era: lessons from the first registry of aortic dissection in China
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Wei-Xun Duan, Wei-Guang Wang, Lin Xia, Chao Xue, Bo Yu, Kai Ren, Wei Yi, Hong-Liang Liang, Xiao-Chao Dong, Jian Zuo, Jin-Cheng Liu, Shi-Qiang Yu, Ding-Hua Yi, Ning-Ning Wang., and On Behalf of the Registry of Aortic Dissection in China (Sino-RAD) investigators
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Medicine - Abstract
Abstract. Background:. Acute type A aortic dissection (ATAAD) and acute type A intramural hematoma (ATAIMH) are life-threatening diseases with high mortality. To better understand their clinical features in the Chinese population, we analyzed the data from the first Registry of Aortic Dissection in China (Sino-RAD) to promote the understanding and management of the diseases. Methods:. All patients with ATAAD and ATAIMH enrolled in Sino-RAD from January 1, 2012 to December 31, 2016 were involved. The data of patients’ selection, history, symptoms, management, outcomes, and postoperation complications were analyzed in the study. The continuous variables were compared using the Student's t test for normal distributions and the Mann-Whitney U test for non-normal distributions. Categorical variables were compared using the Chi-square test or Fisher exact test. Results:. A total of 1582 patients with ATAAD and 130 patients with ATAIMH were included. The mean age of all patients was 48.4 years. Patients with ATAAD were significantly younger than patients with ATAIMH (48.9 years vs. 55.6 years, P
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- 2021
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4. Development of a nomogram to predict progression-free survival in patients with locally advanced renal cell carcinoma
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Ruo-Tao Xiao, Cheng Liu, Bin Yang, Wei He, Chu-Xiao Xu, Zhi-Gang Chen, Lu-Lin Ma, and Yuan-Yuan Ji
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Medicine - Published
- 2021
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5. Sleep-related symptoms in multiple system atrophy: determinants and impact on disease severity
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Jun-Yu Lin, Ling-Yu Zhang, Bei Cao, Qian-Qian Wei, Ru-Wei Ou, Yan-Bing Hou, Kun-Cheng Liu, Xin-Ran Xu, Zheng Jiang, Xiao-Jing Gu, Jiao Liu, Hui-Fang Shang, and Li-Shao Guo
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Medicine - Abstract
Abstract. Background:. Sleep disorders are common but under-researched symptoms in patients with multiple system atrophy (MSA). We investigated the frequency and factors associated with sleep-related symptoms in patients with MSA and the impact of sleep disturbances on disease severity. Methods:. This cross-sectional study involved 165 patients with MSA. Three sleep-related symptoms, namely Parkinson's disease (PD)-related sleep problems (PD-SP), excessive daytime sleepiness (EDS), and rapid eye movement sleep behavior disorder (RBD), were evaluated using the PD Sleep Scale-2 (PDSS-2), Epworth Sleepiness Scale (ESS), and RBD Screening Questionnaire (RBDSQ), respectively. Disease severity was evaluated using the Unified MSA Rating Scale (UMSARS). Results:. The frequency of PD-SP (PDSS-2 score of ≥18), EDS (ESS score of ≥10), and RBD (RBDSQ score of ≥5) in patients with MSA was 18.8%, 27.3%, and 49.7%, respectively. The frequency of coexistence of all three sleep-related symptoms was 7.3%. Compared with the cerebellar subtype of MSA (MSA-C), the parkinsonism subtype of MSA (MSA-P) was associated with a higher frequency of PD-SP and EDS, but not of RBD. Binary logistic regression revealed that the MSA-P subtype, a higher total UMSARS score, and anxiety were associated with PD-SP; that male sex, a higher total UMSARS score, the MSA-P subtype, and fatigue were associated with EDS; and that male sex, a higher total UMSARS score, and autonomic onset were associated with RBD in patients with MSA. Stepwise linear regression showed that the number of sleep-related symptoms (PD-SP, EDS, and RBD), disease duration, depression, fatigue, and total Montreal Cognitive Assessment score were predictors of disease severity in patients with MSA. Conclusions:. Sleep-related disorders were associated with both MSA subtypes and the severity of disease in patients with MSA, indicating that sleep disorders may reflect the distribution and degree of dopaminergic/non-dopaminergic neuron degeneration in MSA.
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- 2021
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6. Identification of a serum three-microRNA signature for cervical cancer diagnosis
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Min-Min Cao, Qing-Xie Liu, Xuan Zou, Xing-Chen Fan, Cheng Liu, Shi-Yu Zhang, Tong-Shan Wang, Chun-Yu Li, Cheng Zhang, Xiang-Nan Geng, Ping Liu, Wei Zhu, and Li-Shao Guo
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Medicine - Published
- 2021
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7. Clinically applicable Gleason grading (GD) system for prostate cancer based on deep learning
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Yun Niu, Can-Cheng Liu, Bing-Lin Zhang, Zhi-Gang Song, Huang Chen, Ping-Ping Liu, Jing-Si Chen, Shu-Hao Wang, Huai-Yin Shi, Ding-Rong Zhong, Jing Ni, and Li-Shao Guo
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Medicine - Published
- 2021
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8. Urinary Biomarkers for Chronic Kidney Disease with a Focus on Gene Transcript
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Lin-Li Lyu, Ye Feng, and Bi-Cheng Liu
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Biomarkers ,Chronic Kidney Disease ,Extracellular Vesicle ,Gene Transcript ,Urine ,Medicine - Abstract
Objective: In the upcoming era of precision medicine, searching for the early, noninvasive biomarkers has been the cornerstone and major challenge in the management of chronic kidney disease (CKD). Urine contains rich biological information which could be the ideal source for noninvasive biomarkers of CKD. This review will discuss the recent advance in urinary biomarker. Data Sources: This review was based on data in articles published in the PubMed databases up to June 20, 2017, with the following keywords: “Chronic kidney disease”, “Biomarker”, and “Urine”. Study Selection: Original articles and important reviews on urinary biomarker were selected for this review. Results: Urinary biomarker studies of CKD mainly focused on urine sediment, supernatant, and urinary extracellular vesicles. The gene transcript (microRNA [miRNA], messenger RNA [mRNA]) biomarkers have been recently shown with diagnostic potential for CKD reflecting kidney function and histological change. However, challenges regarding technique and data analysis need to be resolved before translation to clinic. Conclusions: Different fractions of urine contain rich information for biomarker discovery, among which urine (extracellular vesicles) mRNA, miRNA, might represent promising biomarker for CKD.
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- 2017
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9. Frontolateral Approach Applied to Sellar Region Lesions: A Retrospective Study in 79 Patients
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Hao-Cheng Liu, Zhen Wu, Liang Wang, Xin-Ru Xiao, Da Li, Wang Jia, Li-Wei Zhang, and Jun-Ting Zhang
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Craniopharyngioma ,Meningioma ,Neurosurgery Procedures ,Pituitary Neoplasms ,Skull Base ,Medicine - Abstract
Background: Various surgical approaches for the removal of sellar region lesions have previously been described. This study aimed to evaluate the reliability and safety of the frontolateral approach (FLA) to remove sellar region lesions. Methods: We presented a retrospective study of 79 patients with sellar region lesions who were admitted and operated by the FLA approach from August 2011 to August 2015 in Department of Neurosurgery of Beijing Tian Tan Hospital. We classified FLA into three types, compared the FLA types to the areas of lesion invasion, and analyzed operation bleeding volume, gross total resection (GTR) rate, visual outcome, and mortality. Results: Seventy-nine patients were followed up from 2.9 to 50.3 months with a mean follow-up of 20.5 months. There were 42 cases of meningiomas, 25 cases of craniopharyngiomas, and 12 cases of pituitary adenomas. The mean follow-up Karnofsky Performance Scale was 90.4. GTR was achieved in 75 patients (94.9%). Two patients (2.5%) had tumor recurrence. No patients died perioperatively or during short-term follow-up. Three patients (3.8%) with craniopharyngioma died 10, 12, and 23 months, respectively, after surgery. The operative bleeding volume of this study was no more than that of the other approaches in the sellar region (P = 0.783). In this study, 35 patients (44.3%) had visual improvement after surgery, 38 patients (48.1%) remained unchanged, and three patients' visual outcome (3.8%) worsened. Conclusions: FLA was an effective approach in the treatment of sellar region lesions with good preservation of visual function. FLA classification enabled tailored craniotomies for each patient according to the anatomic site of tumor invasion. This study found that FLA had similar outcomes to other surgical approaches of sellar region lesions.
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- 2016
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10. Surgical Indications of Exploring Optic Canal and Visual Prognostic Factors in Neurosurgical Treatment of Tuberculum Sellae Meningiomas
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Hao-Cheng Liu, E Qiu, Jia-Liang Zhang, Jun Kang, Yong Li, Li-Bin Jiang, and Ji-Di Fu
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Medicine - Abstract
Background:. Tuberculum sellae meningiomas (TSMs) present a special symptom because of the adherence and compression to the optic nerve, optic artery, and the chiasm. A significant number of patients with TSMs appear visual deficits. This study aimed to investigate the surgical indications of exploring the optic canal and visual prognostic factors in the neurosurgical treatment of TSMs. Methods:. Totally 21 patients with TSM, who were operated from September 2007 to August 2011 in the Department of Neurosurgery, Tongren Hospital were enrolled in this study. Results of orbital computed tomography (CT) and magnetic resonance imaging (MRI), visual acuity, Goldmann visual field test, orbital color Doppler flow imaging (CDI) test in these patients were retrospectively analyzed. Results:. Visual deficit and optic canal involvement (OCI) were detected in all the 21 patients. Fourteen patients had bone proliferation within the area of the optic canal. After the operation, visual outcomes were improved in 13 patients, unchanged in 7 patients, and deteriorated in 1 patient. All the 21 patients performed orbital CDI test preoperatively, the results showed that if the peak systolic velocity (PSV) of central retinal artery (CRA) value was ≤8 cm/s, the visual outcome would be better. Conclusions:. The surgical indications of exploring optic canal in TSM cases included: (1) The neuroimaging evidences of OCI (CT and/or MRI); (2) PSV of CRA in orbital CDI test was ≤8 cm/s; (3) visual acuity was below 0.1; (4) visual field deficit. The PSV of CRA in CDI test could be a prognostic factor for visual outcomes of TSMs.
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- 2015
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11. Current Clinical Research of Acute Kidney Injury in China
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Bi-Cheng Liu, Ri-Ning Tang, and Zhi-Hong Liu
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Acute Kidney Injury ,China ,Epidemiology ,Medicine - Published
- 2015
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12. Relative Bioavailability of Rifampicin in Four Chinese Fixed-dose Combinations Compared with Rifampicin in Free Combinations
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Hui Zhu, Shao-Chen Guo, Lan-Hu Hao, Cheng-Cheng Liu, Bin Wang, Lei Fu, Ming-Ting Chen, Lin Zhou, Jun-Ying Chi, Wen Yang, Wen-Juan Nie, and Yu Lu
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Bioequivalence ,Fixed-dose Combination ,Rifampicin ,Tuberculosis ,Medicine - Abstract
Background: Decreases in the bioavailability of rifampicin (RFP) can lead to the development of drug resistance and treatment failure. Therefore, we investigated the relative bioavailability of RFP from one four-drug fixed-dose combination (FDC; formulation A) and three two-drug FDCs (formulations B, C, and D) used in China, compared with RFP in free combinations of these drugs (reference), in healthy volunteers. Methods: Eighteen and twenty healthy Chinese male volunteers participated in two open-label, randomized two-period crossover (formulations A and C) or one three-period crossover (formulations B and D) study, respectively. The washout period between treatments was 7 days. Bioequivalence was assessed based on 90% confidence intervals, according to two one-sided t-tests. All analyses were done with DAS 3.1.5 (Mathematical Pharmacology Professional Committee of China, Shanghai, China). Results: Mean pharmacokinetic parameter values of RFP obtained for formulations A, B, C, and D products were 11.42 ± 3.41 μg/ml, 7.86 ± 5.78 μg/ml, 13.05 ± 6.80 μg/ml, and 16.18 ± 3.87 μg/ml, respectively, for peak plasma concentration (C max ), 91.43 ± 30.82 μg·h−1·ml−1 , 55.49 ± 37.58 μg·h−1·ml−1 , 96.50 ± 47.24 μg·h−1·ml−1 , 101.47 ± 33.07 μg·h−1·ml−1 , respectively, for area under the concentration-time curve (AUC 0-24 h ). Conclusions: Although the concentrations of RFP for formulations A, C, and D were within the reported acceptable therapeutic range, only formulation A was bioequivalent to the reference product. The three two-drug FDCs (formulations B, C and D) displayed inferior RFP bioavailability compared with the reference (Chinese Clinical Trials registration number: ChiCTR-TTRCC-12002451).
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- 2015
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13. Sleep-related symptoms in multiple system atrophy: determinants and impact on disease severity
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Jun-Yu Lin, Ling-Yu Zhang, Bei Cao, Qian-Qian Wei, Ru-Wei Ou, Yan-Bing Hou, Kun-Cheng Liu, Xin-Ran Xu, Zheng Jiang, Xiao-Jing Gu, Jiao Liu, Hui-Fang Shang, and Li-Shao Guo
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Male ,medicine.medical_specialty ,Rapid eye movement sleep ,Excessive daytime sleepiness ,lcsh:Medicine ,Subtype ,REM Sleep Behavior Disorder ,REM sleep behavior disorder ,Severity of Illness Index ,03 medical and health sciences ,0302 clinical medicine ,Atrophy ,stomatognathic system ,Internal medicine ,Severity of illness ,parasitic diseases ,mental disorders ,medicine ,Humans ,Disease severity ,business.industry ,Parkinsonism ,Epworth Sleepiness Scale ,lcsh:R ,Montreal Cognitive Assessment ,General Medicine ,Original Articles ,Multiple system atrophy ,Sleep disorders ,medicine.disease ,nervous system diseases ,Cross-Sectional Studies ,nervous system ,030220 oncology & carcinogenesis ,medicine.symptom ,business ,Sleep ,030217 neurology & neurosurgery - Abstract
Background:. Sleep disorders are common but under-researched symptoms in patients with multiple system atrophy (MSA). We investigated the frequency and factors associated with sleep-related symptoms in patients with MSA and the impact of sleep disturbances on disease severity. Methods:. This cross-sectional study involved 165 patients with MSA. Three sleep-related symptoms, namely Parkinson's disease (PD)-related sleep problems (PD-SP), excessive daytime sleepiness (EDS), and rapid eye movement sleep behavior disorder (RBD), were evaluated using the PD Sleep Scale-2 (PDSS-2), Epworth Sleepiness Scale (ESS), and RBD Screening Questionnaire (RBDSQ), respectively. Disease severity was evaluated using the Unified MSA Rating Scale (UMSARS). Results:. The frequency of PD-SP (PDSS-2 score of ≥18), EDS (ESS score of ≥10), and RBD (RBDSQ score of ≥5) in patients with MSA was 18.8%, 27.3%, and 49.7%, respectively. The frequency of coexistence of all three sleep-related symptoms was 7.3%. Compared with the cerebellar subtype of MSA (MSA-C), the parkinsonism subtype of MSA (MSA-P) was associated with a higher frequency of PD-SP and EDS, but not of RBD. Binary logistic regression revealed that the MSA-P subtype, a higher total UMSARS score, and anxiety were associated with PD-SP; that male sex, a higher total UMSARS score, the MSA-P subtype, and fatigue were associated with EDS; and that male sex, a higher total UMSARS score, and autonomic onset were associated with RBD in patients with MSA. Stepwise linear regression showed that the number of sleep-related symptoms (PD-SP, EDS, and RBD), disease duration, depression, fatigue, and total Montreal Cognitive Assessment score were predictors of disease severity in patients with MSA. Conclusions:. Sleep-related disorders were associated with both MSA subtypes and the severity of disease in patients with MSA, indicating that sleep disorders may reflect the distribution and degree of dopaminergic/non-dopaminergic neuron degeneration in MSA.
- Published
- 2020
14. Clinically applicable Gleason grading (GD) system for prostate cancer based on deep learning
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Yun Niu, Can-Cheng Liu, Bing-Lin Zhang, Zhi-Gang Song, Huang Chen, Ping-Ping Liu, Jing-Si Chen, Shu-Hao Wang, Huai-Yin Shi, Ding-Rong Zhong, Jing Ni, and Li-Shao Guo
- Subjects
Male ,Prostatectomy ,Oncology ,medicine.medical_specialty ,business.industry ,Biopsy ,Deep learning ,MEDLINE ,Gleason grading ,Prostatic Neoplasms ,General Medicine ,medicine.disease ,Prostate cancer ,Deep Learning ,Text mining ,Internal medicine ,Correspondence ,medicine ,Humans ,Medicine ,Artificial intelligence ,Neoplasm Grading ,business - Published
- 2020
15. Neovascularization in the carotid atherosclerotic plaque is not associated with inflammatory cell aggregation
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GAI, Jing-jing, LIU, Hong-bin, LI, Juan, XIE, Lei-xing, ZHAO, Xi-hai, CHENG, Liu-quan, CHEN, Hui-jun, WANG, Yong, YIN, Da-yi, LIU, Jia-jin, and ZHANG, Xiong-wei
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- 2013
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16. Recent trends of study on esophageal variceal bleeding
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CHENG, Liu-fang and LI, Chang-zheng
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- 2010
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17. Treatment of gastric varices by endoscopic sclerotherapy using butyl cyanoacrylate: 10 yearsʼ experience of 635 cases
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CHENG, Liu-fang, WANG, Zhi-qiang, LI, Chang-zheng, CAI, Feng-chun, HUANG, Qi-yang, LINGHU, En-qiang, LI, Wen, CHAI, Guo-jun, SUN, Guo-hui, MAO, Yong-ping, WANG, Yan-mei, LI, Jing, GAO, Ping, and FAN, Tie-yan
- Published
- 2007
18. Frontolateral Approach Applied to Sellar Region Lesions: A Retrospective Study in 79 Patients
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Zhen Wu, Liang Wang, Hao-Cheng Liu, Xinru Xiao, Wang Jia, Liwei Zhang, Junting Zhang, and Da Li
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Adult ,Male ,medicine.medical_specialty ,lcsh:Medicine ,Pituitary neoplasm ,Lesion ,Meningioma ,03 medical and health sciences ,Craniopharyngioma ,0302 clinical medicine ,medicine ,Humans ,Pituitary Neoplasms ,Retrospective Studies ,Skull Base ,medicine.diagnostic_test ,business.industry ,lcsh:R ,Magnetic resonance imaging ,Retrospective cohort study ,General Medicine ,Neurosurgery Procedures ,Middle Aged ,medicine.disease ,Gross Total Resection ,Magnetic Resonance Imaging ,Surgery ,030220 oncology & carcinogenesis ,Original Article ,Female ,Neurosurgery ,medicine.symptom ,business ,030217 neurology & neurosurgery - Abstract
Background: Various surgical approaches for the removal of sellar region lesions have previously been described. This study aimed to evaluate the reliability and safety of the frontolateral approach (FLA) to remove sellar region lesions. Methods: We presented a retrospective study of 79 patients with sellar region lesions who were admitted and operated by the FLA approach from August 2011 to August 2015 in Department of Neurosurgery of Beijing Tian Tan Hospital. We classified FLA into three types, compared the FLA types to the areas of lesion invasion, and analyzed operation bleeding volume, gross total resection (GTR) rate, visual outcome, and mortality. Results: Seventy-nine patients were followed up from 2.9 to 50.3 months with a mean follow-up of 20.5 months. There were 42 cases of meningiomas, 25 cases of craniopharyngiomas, and 12 cases of pituitary adenomas. The mean follow-up Karnofsky Performance Scale was 90.4. GTR was achieved in 75 patients (94.9%). Two patients (2.5%) had tumor recurrence. No patients died perioperatively or during short-term follow-up. Three patients (3.8%) with craniopharyngioma died 10, 12, and 23 months, respectively, after surgery. The operative bleeding volume of this study was no more than that of the other approaches in the sellar region ( P = 0.783). In this study, 35 patients (44.3%) had visual improvement after surgery, 38 patients (48.1%) remained unchanged, and three patients' visual outcome (3.8%) worsened. Conclusions: FLA was an effective approach in the treatment of sellar region lesions with good preservation of visual function. FLA classification enabled tailored craniotomies for each patient according to the anatomic site of tumor invasion. This study found that FLA had similar outcomes to other surgical approaches of sellar region lesions.
- Published
- 2016
19. Differential Innervation of Secretory Coils and Ducts in Human Eccrine Sweat Glands
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Ouyang, Zhan, primary, Li, Hai-Hong, additional, Zhang, Ming-Jun, additional, Xie, Si-Tian, additional, and Cheng, Liu-Hang-Hang, additional
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- 2018
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20. Microinflammation is involved in the dysfunction of arteriovenous fistula in patients with maintenance hemodialysis
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Yugang Wang, Lingjiang Li, Bi Cheng Liu, and Mingming Gao
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Adult ,Male ,medicine.medical_specialty ,Fistula ,medicine.medical_treatment ,Population ,Arteriovenous fistula ,Gastroenterology ,Arteriovenous Shunt, Surgical ,Renal Dialysis ,Internal medicine ,medicine ,Humans ,education ,Dialysis ,Aged ,Inflammation ,Neointimal hyperplasia ,education.field_of_study ,Interleukin-6 ,business.industry ,General Medicine ,Middle Aged ,medicine.disease ,Immunohistochemistry ,Uremia ,Surgery ,C-Reactive Protein ,Female ,Hemodialysis ,Complication ,business - Abstract
BACKGROUND Vascular access (VA) dysfunction is a major clinical complication in the hemodialysis population and has a direct effect on dialysis outcome. This study was conducted to explore the role of microinflammation in the VA dysfunction in maintenance hemodialysis patients. METHODS Forty-seven patients (male 35 and female 12) receiving maintenance hemodialysis were included for this study. They were divided into three groups: group 1 (n = 15), patients with initial hemodialysis and new arteriovenous fistula (AVF); group 2 (n = 18), patients treated with hemodialysis for long term with well-functional VA; group 3 (n = 14), maintenance hemodialysis patients with VA dysfunction. Biochemical parameters and serum tumor necrosis factor-alpha (TNF-alpha), interleukin 6 (IL-6) and monocyte chemoattractant protein-1 (MCP-1) were determined. High-sensitivity C-reactive protein (hs-CRP) was determined by latex-enhanced immuno-nephelometric method. Tissues of radial artery were taken from group 1 and group 3 for the histological study. Expression of CD68 and MCP-1 in the radial artery was determined by immunohistochemistry. RESULTS Serum hs-CRP in group 3 was significantly higher than those in group 1 and group 2 ((7.40 +/- 2.42) mg/L vs (4.21 +/- 1.62) mg/L and (5.04 +/- 3.65) mg/L, P < 0.01 and P < 0.05, respectively). Serum TNF-alpha in group 3 was significantly higher than those in group 1 and group 2 ((64.03 +/- 9.29) pg/ml vs (54.69 +/- 12.39) pg/ml and (54.05 +/- 7.68) pg/ml, P < 0.05 and P < 0.01, respectively). Serum IL-6 in group 3 was also significantly higher than those in group 1 and group 2 ((70.09 +/- 14.53) pg/ml vs (56.43 +/- 10.11) pg/ml and (60.77 +/- 9.70) pg/ml, P < 0.01 and P < 0.05, respectively). Patients in group 3 had a thicker internal layer of vessels than in group 1 ((0.356 +/- 0.056) mm vs (0.111 +/- 0.021) mm, P < 0.01). Expression of CD68 and MCP-1 in the fistula vessel walls in group 3 were much higher than those in group 1 (P < 0.01). Moreover, serum hs-CRP level was positively correlated with the neointimal hyperplasia, the expression of CD68 and MCP-1 in fistula vessel (P < 0.01, respectively). CONCLUSION Microinflammation might be involved in the dysfunction of AVF in patients with maintenance hemodialysis.
- Published
- 2008
21. Soluble expression of active human β-defensin-3 in Escherichia coli and its effects on the growth of host cells
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Gen-Yu Wang, You-Yong Lu, Li-Gang Si, Xi-Cheng Liu, and Li Wz
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chemistry.chemical_classification ,Antimicrobial peptides ,lac operon ,Peptide ,General Medicine ,Biology ,medicine.disease_cause ,Fusion protein ,Microbiology ,law.invention ,chemistry ,law ,Complementary DNA ,Recombinant DNA ,medicine ,Defensin ,Escherichia coli - Abstract
BACKGROUND Human beta-defensin-3 (HBD(3)) is an epithelial peptide that has been demonstrated to have a salt-insensitive broad spectrum of potent antimicrobial activity. Expressing antimicrobial peptides in Escherichia coli (E. coli) is very difficult for it can result in death of the bacterial host cells. Our aim was to establish a prokaryotic system expressing soluble HBD(3) protein and demonstrate the antimicrobial activity of the expressed protein. We then studied whether the host cells would activate the suicide pathways. METHODS We first cloned the complementary DNA coding for the mature chain of HBD(3), inserted it into the vector PGEX-KG then transformed E. coli BL21 (DE3) with the appropriate recombinant plasmid. After induction with 0.5 mmol/L isopropyl-1-thio-beta-D-galactopyranoside (IPTG) the transformed E. coli produced a recombinant glutathione S-transferase and HBD(3) (GST-HBD(3)) fusion protein. The fusion protein was treated with thrombin to produce pure HBD(3) protein then the antimicrobial activity of HBD(3) was evaluated in a liquid microdilution assay. RESULTS The fusion protein GST-HBD(3) was efficiently cleaved by thrombin and yielded HBD(3) that had anti-staphylococcus aureus activity with a minimal inhibitory concentration level of 12.5 microg/ml. The E. coli strain expressing the recombinant protein did not grow slower than the empty vector strain. CONCLUSION Active HBD(3) in E. coli by expressing the recombinant protein GST-HBD(3) could be produced, and suicide did not occur in the E. coli strain expressing the recombinant protein.
- Published
- 2007
22. Synchronous left atrial myxoma and adenosquamous lung carcinoma
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Hong-cheng, Liu, Xiao-feng, Chen, Yu-ming, Yu, and Ge-ning, Jiang
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Heart Neoplasms ,Male ,Neoplasms, Multiple Primary ,Carcinoma, Adenosquamous ,Lung Neoplasms ,Humans ,Heart Atria ,Middle Aged ,Myxoma - Published
- 2013
23. Prevalence, awareness, treatment, and control of hypertension in the non-dialysis chronic kidney disease patients
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Ying, Zheng, Guang-Yan, Cai, Xiang-Mei, Chen, Ping, Fu, Jiang-Hua, Chen, Xiao-Qiang, Ding, Xue-Qing, Yu, Hong-Li, Lin, Jian, Liu, Ru-Juan, Xie, Li-Ning, Wang, Zhao-Hui, Ni, Fu-You, Liu, Ai-Ping, Yin, Chang-Ying, Xing, Li, Wang, Wei, Shi, Jian-She, Liu, Ya-Ni, He, Guo-Hua, Ding, Wen-Ge, Li, Guang-Li, Wu, Li-Ning, Miao, Nan, Chen, Zhen, Su, Chang-Lin, Mei, Jiu-Yang, Zhao, Yong, Gu, Yun-Kai, Bai, Hui-Min, Luo, Shan, Lin, Meng-Hua, Chen, Li, Gong, Yi-Bin, Yang, Xiao-Ping, Yang, Ying, Li, Jian-Xin, Wan, Nian-Song, Wang, Hai-Ying, Li, Chun-Sheng, Xi, Li, Hao, Yan, Xu, Jing-Ai, Fang, Bi-Cheng, Liu, Rong-Shan, Li, Rong, Wang, Jing-Hong, Zhang, Jian-Qin, Wang, Tan-Qi, Lou, Feng-Min, Shao, Feng, Mei, Zhi-Hong, Liu, Wei-Jie, Yuan, Shi-Ren, Sun, Ling, Zhang, Chun-Hua, Zhou, Qin-Kai, Chen, Shun-Lian, Jia, Zhi-Feng, Gong, Guang-Ju, Guan, Tian, Xia, and Liang-Bao, Zhong
- Subjects
Adult ,Male ,Hypertension ,Prevalence ,Humans ,Female ,Awareness ,Middle Aged ,Renal Insufficiency, Chronic ,Aged - Abstract
Data on the epidemiology of hypertension in Chinese non-dialysis chronic kidney disease (CKD) patients are limited. The aim of the present study was to investigate the prevalence, awareness, treatment, and control of hypertension in the non-dialysis CKD patients through a nationwide, multicenter study in China.The survey was performed in 61 tertiary hospitals in 31 provinces, municipalities, and autonomous regions in China (except Hong Kong, Macao, and Taiwan). Trained physicians collected demographic and clinical data and measured blood pressure (BP) using a standardized protocol. Hypertension was defined as systolic BP ≥ 140 mmHg and/or diastolic BP ≥ 90 mmHg, and/or use of antihypertensive medications. BP140/90 mmHg and130/80 mmHg were used as the 2 thresholds of hypertension control. In multivariate logistic regression with adjustment for sex and age, we analyzed the association between CKD stages and uncontrolled hypertension in non-dialysis CKD patients.The analysis included 8927 non-dialysis CKD patients. The prevalence, awareness, and treatment of hypertension in non-dialysis CKD patients were 67.3%, 85.8%, and 81.0%, respectively. Of hypertensive CKD patients, 33.1% and 14.1% had controlled BP to140/90 mmHg and130/80 mmHg, respectively. With successive CKD stages, the prevalence of hypertension in non-dialysis CKD patients increased, but the control of hypertension decreased (P0.001). When the threshold of BP130/80 mmHg was considered, the risk of uncontrolled hypertension in CKD 2, 3a, 3b, 4, and 5 stages increased 1.3, 1.4, 1.4, 2.5, and 4.0 times compared with CKD 1 stage, respectively (P0.05). Using the threshold of140/90 mmHg, the risk of uncontrolled hypertension increased in advanced stages (P0.05).The prevalence of hypertension Chinese non-dialysis CKD patients was high, and the hypertension control was suboptimal. With successive CKD stages, the risk of uncontrolled hypertension increased.
- Published
- 2013
24. Application of systems biology to the study of chronic kidney disease
- Author
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Yu-Han, Cao, Lin-Li, Lü, Jian-Dong, Zhang, and Bi-Cheng, Liu
- Subjects
Gene Expression Profiling ,Systems Biology ,Computational Biology ,Humans ,Renal Insufficiency, Chronic ,Genome-Wide Association Study - Abstract
Chronic kidney disease (CKD) is a major public health problem that affects about 10% of the general population. Current approaches to characterize the category and progression of CKD are normally based on renal histopathological results and clinical parameters. However, this information is not sufficient to predict CKD progression risk reliably or to guide preventive interventions. Nowadays, the appearance of systems biology has brought forward the concepts of "-omics" technologies, including genomics, transcriptomics, proteomics, and metabolomics. Systems biology, together with molecular analysis approaches such as microarray analysis, genome-wide association studies (GWAS), and serial analysis of gene expression (SAGE), has provided the framework for a comprehensive analysis of renal disease and serves as a starting point for generating novel molecular diagnostic tools for use in nephrology. In particular, analysis of urinary mRNA and protein levels is rapidly evolving as a non-invasive approach for CKD monitoring. All these systems biological molecular approaches are required for application of the concept of "personalized medicine" to progressive CKD, which will result in tailoring therapy for each patient, in contrast to the "one-size-fits-all" therapies currently in use.
- Published
- 2012
25. Influence of irbesartan on the urinary excretion of cytokines in patients with chronic kidney disease
- Author
-
Jie, Ni, Hai-Quan, Huang, Lin-Li, Lü, Min, Zheng, and Bi-Cheng, Liu
- Subjects
Cross-Over Studies ,Creatinine ,Biphenyl Compounds ,Chronic Disease ,Cytokines ,Humans ,Tetrazoles ,Kidney Diseases ,Irbesartan ,Prospective Studies ,Angiotensin II Type 1 Receptor Blockers - Abstract
The non-hemodynamic effects of angiotensin receptor blocker (ARB) in the delay of progression of chronic kidney disease (CKD) remain unclear. In this study, we investigated the influence of irbesartan on the urinary excretion of cytokines in patients with CKD.In this randomized perspective clinical trial, different doses of irbesartan (150 mg/d and 300 mg/d) were given to two groups of patients in a cross-over design. Blood pressure (BP), creatinine clearance (Ccr) and 24-hour proteinuria were examined. Urinary excretion of cytokines was determined by human inflammatory cytokine antibody array. A two-fold change in spot intensity was considered significant.Urinary excretion of cytokines (granulocyte colony stimulating factor (GCSF), intercellular cell adhesion molecule-1 (ICAM-1), interferon γ (IFN-γ), interleukin 1β (IL-1b), IL-2, IL-6, IL-8, IL-11, IL-15 and macrophage inflammatory protein 1d (MIP-1d)) in group B (irbesartan 300 mg/d) was significantly decreased in comparison to group A (irbesartan 150 mg/d) after 8-week treatment. In group A, 8 weeks of treatment induced a two- to nine-fold reduction in urinary cytokine levels (GCSF, GM-CSF, IFN-γ, IL-1a, IL-11, IL-12p40, MCP-2, MIP-1a), while increasing the dosage to 300 mg/d further decreased the excretion of GCSF, GM-CSF, IL-12p40, MCP-2 and MIP-1a by week 18. There was no significant difference in BP or Ccr between the two groups. However, 24-hour proteinuria was significantly reduced in both groups, and in group A the reduction was dose dependent.Irbesartan offers additional renoprotection in a dose-dependent manner by reducing pro-inflammatory cytokines excretion in the urine of CKD patients.
- Published
- 2012
26. Inhibition of integrin-linked kinase by angiotensin II receptor antagonist, irbesartan attenuates podocyte injury in diabetic rats
- Author
-
Hou-yong, Dai, Min, Zheng, Ri-ning, Tang, Kun-ling, Ma, Jie, Ni, and Bi-cheng, Liu
- Subjects
Enzyme Activation ,Male ,Angiotensin Receptor Antagonists ,Podocytes ,Rats, Inbred SHR ,Biphenyl Compounds ,Animals ,Tetrazoles ,Irbesartan ,Protein Serine-Threonine Kinases ,Diabetes Mellitus, Experimental ,Rats - Abstract
Integrin-linked kinase (ILK) dysregulation is involved in the progression of diabetic nephropathy (DN). The aim of this study was to investigate the effects of angiotensin II receptor blocker (ARB), irbesartan, on ILK expression and podocyte injury in DN.DN was induced by the combined feeding of high-sucrose, high-fat diet and intra-peritoneal injection of low dose of streptozotocin (35 mg/kg) in spontaneously hypertensive rats. Diabetic rats were treated with irbesartan (50 mg×kg(-1)×d(-1)) by gavage for 8 weeks. The renal morphologic changes and podocyte injury were investigated by light and electron microscopy, and the ILK expression was evaluated by real-time RT-PCR and Western blotting analysis.Diabetic rats exhibited with the similar clinical feature of type 2 DN. Morphologically, they were characterized by expansion of mesangial matrix, loss of podocyte and podocyte injury. Impressively, compared to controls, the ILK expression in diabetic rats were upregulated, which were positively correlated with both podocyte injury and albuminuria. Irbesartan significantly prevented ILK overexpression, along with the amelioration of podocyte injury and albuminuria.ILK plays an important role in mediating podocyte injury in DN; irbesartan inhibits ILK upregulation and attenuates podocyte injury, which might offer a new insight into the role of ARB in preventing DN progression.
- Published
- 2012
27. A new rat model of glaucoma induced by intracameral injection of silicone oil and electrocoagulation of limbal vessels
- Author
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Xue-Qian, Guo, Bei, Tian, Zhi-Cheng, Liu, Wen-Bin, Wei, Yong, Tao, Shi-Jie, Sun, and Yu, Zhang
- Subjects
Male ,Disease Models, Animal ,Electrocoagulation ,Animals ,Silicone Oils ,Glaucoma ,Limbus Corneae ,Rats, Wistar ,Rats - Abstract
A satisfied glaucoma model is absent now. The aim of this study was to evaluate the effect of a combination of intracameral injection of silicone oil and electrocoagulation of corneal limbal vessels and episcleral veins in the rats to establish glaucoma model.Operation was performed in each of the left eyes of 90 adult male rats. Right eyes were used as controls. Measurement of intraocular pressure (IOP) was performed with an applanation tonometer (Tono-Pen). Retinal ganglion cells (RGCs) were retrogradely labeled by applying FluoroGold onto the bilateral superior colliculus.During the follow-up (24 weeks), the IOP of the study eyes was significantly higher (P0.05) than the control eyes (at final examination, IOP of control eyes was (13.4 ± 1.0) mmHg and IOP of study eyes was (16.1 ± 1.8) mmHg). Correspondingly, at 24 weeks after operation, the RGCs density of the study eyes (2286.11 ± 290.45/mm(2)) was significantly lower than the control eyes (2626.46 ± 164.85/mm(2), P0.01). In the operated eyes, histological examination showed excavation of optic disc and increased neuroglial cells in the optic nerve, reduced thickness of retina and diminution of retinal ganglion cells, and atrophy of ciliary body and iris. Notably, the anterior chamber angle of the operated eye remained open.A combination of intracameral injection of silicone oil and electrocoagulation of corneal limbal vessels and episcleral veins may establish a reliable glaucoma model for further research.
- Published
- 2011
28. Titanium internal fixation system used for sternum reconstruction after resection of chondrosarcoma
- Author
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Zheng-cheng, Liu and Heng, Zhao
- Subjects
Titanium ,Fracture Fixation, Internal ,Sternum ,Chondrosarcoma ,Humans ,Female ,Aged - Published
- 2010
29. Novel biomarkers for progression of chronic kidney disease
- Author
-
Bi-Cheng, Liu and Lin-Li, Lü
- Subjects
Proteomics ,Membrane Glycoproteins ,Lipocalin-2 ,Proto-Oncogene Proteins ,Cytokines ,Humans ,Kidney Failure, Chronic ,Receptors, Virus ,Hepatitis A Virus Cellular Receptor 1 ,Fatty Acid-Binding Proteins ,Biomarkers ,Lipocalins ,Acute-Phase Proteins - Published
- 2010
30. Hemodynamic effects of stenting on wide-necked intracranial aneurysms
- Author
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Yi-sen, Zhang, Xin-jian, Yang, Sheng-zhang, Wang, Ai-ke, Qiao, Jia-liang, Chen, Kun-ya, Zhang, Zhi-cheng, Liu, Yu-jing, Zhao, Ying, Zhang, Bin, Luo, and Chuan-hui, Li
- Subjects
Hydrodynamics ,Humans ,Intracranial Aneurysm ,Stents ,Embolization, Therapeutic - Abstract
Stent placement has been widely used to assist coiling in cerebral aneurysm treatments. The present study aimed to investigate the hemodynamic effects of stenting on wide-necked intracranial aneurysms.Three idealized plexiglass aneurismal models with different geometries before and after stenting were created, and their three-dimensional computational models were constructed. Flow dynamics in stented and unstented aneurismal models were studied using in vitro flow visualization and computational fluid dynamics (CFD) simulations. In addition, effects of stenting on flow dynamics in a patient-specific aneurysm model were also analyzed by CFD.The results of flow visualization were consistent with those obtained with CFD simulations. Stent deployment reduced vortex inside the aneurysm and its impact on the aneurysm sac, and decreased wall shear stress on the sac. Different aneurysm geometries dictated fundamentally different hemodynamic patterns and outcomes of stenting.Stenting across the neck of aneurysms improves local blood flow profiles. This may facilitate thrombus formation in aneurysms and decrease the chance of recanalization.
- Published
- 2010
31. Cerebral artery evaluation of dual energy CT angiography with dual source CT
- Author
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Rui, Ma, Cheng, Liu, Kai, Deng, Shao-juan, Song, Dao-ping, Wang, and Ling, Huang
- Subjects
Adult ,Male ,Young Adult ,Adolescent ,Angiography ,Humans ,Radiographic Image Interpretation, Computer-Assisted ,Female ,Cerebral Arteries ,Middle Aged ,Tomography, X-Ray Computed ,Aged - Abstract
Conventional computed tomography angiography (CTA) is time consuming, user-dependent and has poor image quality in skull base region. This study assessed the feasibility of a new method, dual energy CTA for depicting the cerebral artery.Phantom scan was done with head CTA sequences on dual source CT and 64 spiral CT for radiation dose calculation. Dual energy CTA was done with dual source CT on 36 patients who were suspected of having cerebral vascular disease. Three series axial images in 0.75 mm thick, 0.4 mm increment were acquired, which were named with 80 kV, 140 kV and merged images; 80 kV and 140 kV images were transferred into dual energy software, and maximum intensity projection (MIP) image was generated quickly by dual energy bone remove (DEBR group); merged images were transferred into In Space software to acquire MIP image through manual conventional bone remove (CoBR group). Post processing time and reading time were compared. Image qualities of the two groups were compared, mainly focusing on skull base segments of internal carotid artery and bone subtraction. ANOVA and SNK tests were applied for radiation dose comparison. Student's t test and Wilcoxon rank sum test were applied for assessing differences between data for significance. Cohen's kappa was used for interobserver agreement.Radiation dose of phantom scan showed dual energy CTA was between digital bone subtraction and conventional CTA. The post processing time and reading time were much shorter in DEBR than CoBR, and image quality in skull base was much higher in DEBR than CoBR (P0.01). There was no significant difference for suprasellar vessels between two groups (P0.5). Interobserver agreement for all vessel segments was excellent (kappa = 0.97).Dual energy CTA is a reliable, new modality for depicting cerebral artery, overcoming the limitation of conventional CTA in the skull base region. It can save much time in post processing and reading than conventional CTA.
- Published
- 2010
32. Craniotomy with endoscopic assistance in the treatment of nasopharygeal fibroangioma
- Author
-
Ji-di, Fu, Hao-cheng, Liu, Shang-feng, Zhao, Jia-liang, Zhang, Yong, Li, Xin, Ni, and Chun-jiang, Yu
- Subjects
Adult ,Male ,Radiography ,Young Adult ,Adolescent ,Child, Preschool ,Humans ,Endoscopy ,Nasopharyngeal Neoplasms ,Fibroma ,Child ,Magnetic Resonance Imaging ,Craniotomy - Abstract
Nasopharygeal fibroangioma (NPF) can be approached through lateral rhinotomy, the middle skull fossa approach and the transcranial-facial combined approach. It is complicated and thus results in more insults, and when adopted, the total resection rate of tumor is still low. The nasal endoscope is minimally invasive, the dead angles of a craniotomy, such as sphenoid sinus, maxillary sinus, and nasopharynx are easily approached by an endoscope. Lateral rhinotomy have to make facial incision and affects maxillary bone development. We combined the craniotomy and endoscopic approach intending to take advantages of the two approaches.Twelve NPF patients who underwent craniotomy with endoscopic assistance from March 2002 to July 2008 at the Beijing Tongren Hospital were selected. All patients were male. Their ages ranged from 11 to 33 years. The main symptoms were visual deterioration, exophthalmos, nasal obstruction, epistaxis and pharynx nasalis neoplasm. The diagnosis was based on CT, MRI and digital subtraction angiography (DSA). All patients had intracranial encroachment and all underwent DSA and embolism treatment were taken before surgery. Seven patients had a pterional craniotomy, five had a frontal-temporal-orbital-zygomatic craniotomy. Most of the tumor was resected piecemeal, then removed through the sphenoidal sinus. Finally, using an endoscope in the nasal cavity, tumor in nasal cavity was resected and removed through the sphenoidal sinus, observing the dead angle of the craniotomy and confirming that sinus drainage was unobstructed.The tumor was removed completely in 11 patients and partially resected in one patient because of hemorrhage. One patient had an infection after the operation and one patient had cerebrospinal rhinorrhea 3 years after surgery that was remediated by endoscopic repair.Craniotomy with endoscopic assistance in the treatment of NPF was minimally invasive, safe and efficient, and avoided facial incision.
- Published
- 2010
33. Levels and dynamic changes of serum fibroblast growth factor 23 in hypophosphatemic rickets/osteomalacia
- Author
-
Wei-bo, Xia, Yan, Jiang, Mei, Li, Xiao-ping, Xing, Ou, Wang, Ying-ying, Hu, Hua-bing, Zhang, Huai-cheng, Liu, Xun-wu, Meng, and Xue-ying, Zhou
- Subjects
Adult ,Male ,Adolescent ,Enzyme-Linked Immunosorbent Assay ,Middle Aged ,Phosphates ,Fibroblast Growth Factors ,Fibroblast Growth Factor-23 ,Young Adult ,Calcitriol ,Child, Preschool ,Osteomalacia ,Humans ,Female ,Familial Hypophosphatemic Rickets ,Child - Abstract
Hypophosphatemic rickets/osteomalacia is a group of diseases characterised by defective mineralization of bone due to hypophosphatemia and low 1,25-dihydroxy vitamin D. To explore the role of fibroblast growth factor 23 (FGF-23) in the regulation of phosphate homeostasis, we measured the circulating concentrations of this growth factor in healthy individuals and in patients with hypophosphatemic rickets/osteomalacia.Nineteen patients with hypophosphatemic rickets/osteomalacia were included in hypophosphatemic group (HP, 12 female and 7 male, mean age was 30 years), and 19 healthy age-matched individuals served as the control group. Full length FGF-23 fragments were measured by two-site enzyme-linked immunosorbent assay.Mean FGF-23 concentrations were significantly higher in the HP group ((87.4 +/- 43.6) pg/ml) compared with the control group ((19.2 +/- 6.16) pg/ml; P0.001). In 1 patient with tumour-induced osteomalacia, serum FGF-23 concentrations were 84.1 pg/ml; these concentrations were normalized 2 hours after a hemangiopericytoma resection (7.8 pg/ml). Subsequently, serum 1,25(OH)(2) vitamin D3 concentrations significantly increased from 21.3 pg/ml to 89.3 pg/ml, and serum phosphorus levels were normalized.Serum FGF-23 concentrations were markedly elevated in patients with hypophosphatemic rickets. FGF-23 plays an important role in the pathogenesis of hypophosphatemic rickets/osteomalacia.
- Published
- 2010
34. Treatment of hypocalcemia caused by hypoparathyroidism or pseudohypoparathyroidism with domestic-made calcitriol: a prospective and self-controlled clinical trial
- Author
-
Ou, Wang, Xiao-ping, Xing, Xun-wu, Meng, Wei-bo, Xia, Mei, Li, Yan, Jiang, Ying-ying, Hu, and Huai-cheng, Liu
- Subjects
Adult ,Male ,Calcitriol ,Hypocalcemia ,Hypoparathyroidism ,Pseudohypoparathyroidism ,Humans ,Calcium ,Female ,Prospective Studies ,Middle Aged - Abstract
Parathyroid hormone deficiency or resistance may cause hypocalcemia with related symptoms and signs. Lifelong treatment of calcium combined with vitamin D or its metabolites is always necessary for these patients. Here we reported a prospective and open-label trial to investigate the efficacy and safety of domestic-made calcitriol in treatment of hypocalcemia caused by hypoparathyroidism or pseudohypoparathyroidism.Twenty-four patients with confirmed hypoparathyroidism or pseudohypoparathyroidism aged (36.5 +/- 11.0) years old were studied. Among them, 16 patients had idiopathic hypoparathyroidism, 2 had pseudohypoparathyroidism and 6 had hypoparathyroidism secondary to cervical surgery. Serum calcium levels were lower than 1.88 mmol/L. Oral calcitriol was administered twice or three times with elemental calcium 1.2 g per day. All patients were followed every 4 weeks throughout the 12-week period. Dose adjustments of calcitriol were based on serum and urinary calcium levels and symptoms of hypocalcemia.Twenty patients were included by the end of this study. Muscular weakness, cramps, extremity paresthesia, Chovestek's sign and Trousseau's sign were relieved in 76.9%, 100%, 94.4%, 93.3% and 78.9% of patients, respectively. Serum calcium, plasma ionized calcium and serum phosphorus levels were (1.54+/-0.25) mmol/L, (0.64+/-0.10) mmol/L and (2.00+/-0.46) mmol/L at baseline, and reached (2.20+/-0.20) mmol/L, (0.95+/-0.06) mmol/L and (1.68+/-0.25) mmol/L (P0.01) at the 12th week of treatment, respectively. Eighty percent of patients were assessed as effective and 20% as partly effective. Three, four and eight patients had hypercalciuria at the 4th, 8th and 12th week of treatment, respectively, which were reduced by thiazide diuretics. The final dose of calcitriol was (1.09+/-0.50) microg/d.Calcitriol combined with calcium can be used in treatment of hypocalcemia caused by hypoparathyroidism or pseudohypoparathyroidism effectively and safely. Serum and urinary calcium levels should be monitored during the course of the therapy.
- Published
- 2009
35. Effects of granulocyte colony-stimulating factor on repair of injured canine arteries
- Author
-
Que-Lin Mei, Hong-Jian Yu, Yan-Hao Li, Zai-zhong Chen, Jian-yong Yang, and Peng-cheng Liu
- Subjects
Male ,Pathology ,medicine.medical_specialty ,medicine.medical_treatment ,Dogs ,Renal Artery ,Restenosis ,Angioplasty ,White blood cell ,Granulocyte Colony-Stimulating Factor ,Medicine ,Animals ,Progenitor cell ,Neointimal hyperplasia ,Hyperplasia ,business.industry ,General Medicine ,medicine.disease ,Hematopoietic Stem Cell Mobilization ,Recombinant Proteins ,Granulocyte colony-stimulating factor ,Stenosis ,medicine.anatomical_structure ,Bone marrow ,business ,Tunica Intima - Abstract
BACKGROUND Endothelial progenitor cells (EPCs) derived from bone marrow may differentiate into endothelial cells and participate in endothelial repair. These cells can be mobilized into peripheral blood by cytokines, including granulocyte colony-stimulating factor (G-CSF). In the present study, we investigated the effects of G-CSF on neointimal formation and restenosis in a canine model of arterial balloon injury. METHODS Sixteen male beagle dogs were injected subcutaneously with 20 microg x kg(-1) x d(-1) recombinant human G-CSF (n = 8) or normal saline (n = 8) for 1 week. On the fifth day of treatment, the dogs underwent renal arterial angioplasty. At 8 weeks after arterial balloon injury, angiographic observations were made and injured arteries were processed for morphometric analysis of neointimal formation. RESULTS Peripheral white blood cell counts were increased by 3.34-fold compared to baseline on the fifth day of administration of G-CSF. Angiographies revealed that one stenosis had occurred among the eight injured renal arteries from dogs treated with G-CSF, whereas all injured renal arteries from dogs treated with normal saline remained patent. The mean extent of stenosis among injured arteries was 18.3% +/- 17.9% in the G-CSF treated group compared to 12.5% +/- 7.6% in the saline treated control group (P = 0.10). G-CSF treatment slightly increased neointimal thickness (0.42 +/- 0.15 mm vs 0.25 +/- 0.06 mm, P = 0.08) with an intima to media ratio of 0.83 +/- 0.49 vs 0.54 +/- 0.18 (P = 0.11). CONCLUSIONS G-CSF treatment does not attenuate neointimal hyperplasia and restenosis formation in a canine model of renal arterial injury, suggesting that the therapeutic strategy for preventing restenosis by stem cell mobilization should be investigated further.
- Published
- 2008
36. Epithelial to mesenchymal transition in the progression of tubulointerstitial fibrosis
- Author
-
Bi-cheng Liu and Min-xia Li
- Subjects
Pathology ,medicine.medical_specialty ,Epithelium ,Immediate-Early Proteins ,Mesoderm ,Fibrosis ,Renal fibrosis ,Medicine ,Animals ,Humans ,Integrin-linked kinase ,Epithelial–mesenchymal transition ,biology ,business.industry ,Mechanism (biology) ,Disease progression ,Connective Tissue Growth Factor ,General Medicine ,medicine.disease ,Transforming Growth Factors ,embryonic structures ,biology.protein ,Cancer research ,Tubulointerstitial fibrosis ,Disease Progression ,Intercellular Signaling Peptides and Proteins ,Nephritis, Interstitial ,business ,Transforming growth factor - Abstract
Objective To review the mechanisms of epithelial to mesenchymal transition (EMT) and its role in the progression of tubulointerstitial fibrosis. Data sources The data used in this review were obtained mainly from the studies of EMT reported from 2000-2006. Study selection Relevant articles on studies of EMT in tubulointerstitial fibrosis were selected. Data were mainly extracted from the 45 articles listed in the reference section of this review. Results The process of EMT has gained wide recognition as candidate mechanism in progression of chronic fibrotic disorders. New markers were identified and facilitate the observation of EMT. EMT is regulated by many factors through activation of kinase-dependent signaling cascades. Recent findings suggest that EMT is a reversible process, which can be controlled by factors for their epithelial inducing activities. Conclusion Remarkable progresses of EMT research have been made recently. Preventing or reversing EMT is a promising strategy against renal fibrosis.
- Published
- 2007
37. Soluble expression of active human beta-defensin-3 in Escherichia coli and its effects on the growth of host cells
- Author
-
Li-Gang, Si, Xi-Cheng, Liu, You-Yong, Lu, Gen-Yu, Wang, and Wen-Mei, Li
- Subjects
Staphylococcus aureus ,DNA, Complementary ,beta-Defensins ,Reverse Transcriptase Polymerase Chain Reaction ,Recombinant Fusion Proteins ,Molecular Sequence Data ,Thrombin ,Sequence Analysis, DNA ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,Escherichia coli ,Humans ,Electrophoresis, Polyacrylamide Gel ,Amino Acid Sequence ,Glutathione Transferase ,Plasmids - Abstract
Human beta-defensin-3 (HBD(3)) is an epithelial peptide that has been demonstrated to have a salt-insensitive broad spectrum of potent antimicrobial activity. Expressing antimicrobial peptides in Escherichia coli (E. coli) is very difficult for it can result in death of the bacterial host cells. Our aim was to establish a prokaryotic system expressing soluble HBD(3) protein and demonstrate the antimicrobial activity of the expressed protein. We then studied whether the host cells would activate the suicide pathways.We first cloned the complementary DNA coding for the mature chain of HBD(3), inserted it into the vector PGEX-KG then transformed E. coli BL21 (DE3) with the appropriate recombinant plasmid. After induction with 0.5 mmol/L isopropyl-1-thio-beta-D-galactopyranoside (IPTG) the transformed E. coli produced a recombinant glutathione S-transferase and HBD(3) (GST-HBD(3)) fusion protein. The fusion protein was treated with thrombin to produce pure HBD(3) protein then the antimicrobial activity of HBD(3) was evaluated in a liquid microdilution assay.The fusion protein GST-HBD(3) was efficiently cleaved by thrombin and yielded HBD(3) that had anti-staphylococcus aureus activity with a minimal inhibitory concentration level of 12.5 microg/ml. The E. coli strain expressing the recombinant protein did not grow slower than the empty vector strain.Active HBD(3) in E. coli by expressing the recombinant protein GST-HBD(3) could be produced, and suicide did not occur in the E. coli strain expressing the recombinant protein.
- Published
- 2007
38. Clinical features of renal artery stenosis in elderly patients
- Author
-
Gen-Shan Ma, Yan-Li Wang, Bi-Cheng Liu, Li-Qun Ren, and Ri-Ning Tang
- Subjects
medicine.medical_specialty ,medicine.diagnostic_test ,business.industry ,Renal Artery Obstruction ,Coronary Disease ,General Medicine ,Coronary disease ,Renal artery stenosis ,medicine.disease ,Surgery ,Risk Factors ,Internal medicine ,Angiography ,Hypertension ,medicine ,Cardiology ,Humans ,Renal Insufficiency ,business ,Aged - Published
- 2007
39. Connective tissue growth factor is associated with the early renal hypertrophy in uninephrectomized diabetic rats
- Author
-
Dong-Dong Luo, Bi-Cheng Liu, Hai-Quan Huang, Dian-Ge Liu, Kun-Ling Ma, and Hong Liu
- Subjects
Male ,medicine.medical_specialty ,Renal Hypertrophy ,urologic and male genital diseases ,Kidney ,Nephrectomy ,Streptozocin ,Muscle hypertrophy ,Diabetes Mellitus, Experimental ,Immediate-Early Proteins ,Diabetic nephropathy ,Rats, Sprague-Dawley ,Diabetes mellitus ,Internal medicine ,medicine ,Renal fibrosis ,Albuminuria ,Animals ,business.industry ,Glomerular basement membrane ,Connective Tissue Growth Factor ,General Medicine ,Hypertrophy ,medicine.disease ,Rats ,CTGF ,medicine.anatomical_structure ,Endocrinology ,Intercellular Signaling Peptides and Proteins ,business ,Cyclin-Dependent Kinase Inhibitor p27 - Abstract
BACKGROUND Renal hypertrophy has been regarded as the early feature of diabetic nephropathy (DN), which may eventually lead to proteinuria and renal fibrosis. However, the exact mechanism of renal hypertrophy is still unclear. The aim of this study was to investigate the possible association of connective tissue growth factor (CTGF) with renal hypertrophy in uninephrectomized diabetic rats. METHODS Seventy-two Sprague-Dawley (SD) rats were randomly divided into two groups: control group (group C, n = 32) and diabetic nephropathy (group DN, n = 40). Each group was re-divided into 4 subgroups according to the experimental period. The rats were sacrificed at 1, 2, 4, and 8 weeks respectively after induction of diabetes. Diabetes was induced by intraperitoneal injection of streptozotocin (STZ) after rats had received uninephrectomy. Blood glucose (BG), body weight (BW), 24-h urinary albumin excretion (24hUalb), kidney weight (KW), KW/BW, glomerular tuft area (AG), glomerular tuft volume (VG), proximal tubular area (AT) at each time point, the width of glomerular basement membrane (GBM) and tubular basement membrane (TBM) at week 8 were measured when the rats were sacrificed. Renal expression of CTGF and p27kip1 were detected by immunohistochemical staining. The relationship between CTGF expression and increasing of VG and AT was analyzed. RESULTS There was a significant increase of 24hUalb, KW, and KW/BW from week 1 onward in diabetic rats compared to those in group C (P < 0.05, respectively), diabetic rats also had a significant increase of AG, VG, and AT from week 1 onward. It was also shown that diabetic rats had a thickening of GBM [(245.7 +/- 103.0) nm vs (121.8 +/- 19.1) nm, P < 0.01] and TBM [(767.7 +/- 331.1) nm vs (293.0 +/- 110.5) nm, P < 0.01] at week 8. There was a weak expression for CTGF and p27kip1 in normal glomeruli and tubuli, while a significant increasing expression of CTGF and p27kip1 was found in glomeruli and tubuli in diabetic kidney from week 1 onward (P < 0.05, respectively), and the extent of CTGF expression was positively correlated with AG (r = 0.92, P < 0.05), VG (r = 0.86, P < 0.05), AT (r = 0.94, P < 0.01) and positively correlated with the expression of p27kip1 (r = 0.96, P < 0.01). CONCLUSION The expression of CTGF increases in diabetic rat kidney at the early stage, which might be an important mediator of renal hypertrophy through arresting cell cycling.
- Published
- 2006
40. Effects of irbesartan on the expression of matrix metalloproteinase-2/tissue inhibitor of metalloproteinase-2 in streptozotocin-induced diabetic rat kidney
- Author
-
Bi-cheng, Liu, Yan, Xu, Kun-ling, Ma, Hai-quan, Huang, Lian-fang, Yin, and Dian-ge, Liu
- Subjects
Male ,Tissue Inhibitor of Metalloproteinase-2 ,Biphenyl Compounds ,Tetrazoles ,Hypertrophy ,Irbesartan ,Kidney ,Immunohistochemistry ,Streptozocin ,Diabetes Mellitus, Experimental ,Rats ,Rats, Sprague-Dawley ,Creatinine ,Animals ,Matrix Metalloproteinase 2 ,Angiotensin II Type 1 Receptor Blockers - Published
- 2005
41. Effect of irbesartan on angiotensin II-induced hypertrophy of human proximal tubular cells
- Author
-
Bi-Cheng, Liu, Jing, Sun, Qi, Chen, Dong-Dong, Luo, Kun-Ling, Ma, and Xiong-Zhong, Ruan
- Subjects
Kidney Tubules, Proximal ,Angiotensin II ,Protein Biosynthesis ,Biphenyl Compounds ,Cell Cycle ,Humans ,Tetrazoles ,Hypertrophy ,Irbesartan ,Angiotensin II Type 1 Receptor Blockers ,Cells, Cultured - Abstract
Intrarenal activation of the renin angiotensin system (RAS) plays an important role in mediating renal fibrosis. Both angiotensin converting enzyme inhibitors (ACEIs) and angiotensin II (AngII) receptor antagonists have been shown to exert a protective role against diabetic and non-diabetic nephropathy. However, the exact mechanism of how blocking local RAS prevents renal fibrosis is unclear. The present study was to investigate the influence of a new AngII receptor antagonist, irbesartan (Irb), on AngII-induced hypertrophy in human proximal tubular cell line (HK-2).The cell line, HK-2, was grown in Dulbeccos's Modified Eagle's Medium containing 10% heat-inactivated fetal calf serum. After rested in serum-free medium for 24 hours, the effects of Irb on AngII (10(-7) mol/L)-induced [(3)H]-leucine incorporation, total protein content (measured by the Coomassie brilliant blue G250 method), and change in cell size (determined by scanning electron microscopy) were observed. The influence of Irb on the cell cycle was analyzed by fluorescence activated cell sorter (FACS) flow cytometry.AngII induced cell hypertrophy in a time and dose dependent manner. Stimulation of cells with AngII for 48 hours resulted in a increase in [(3)H]-leucine incorporation [0 hour: (5584 +/- 1016) cpm/10(5) cells vs 48 hours: (10741 +/- 802) cpm/10(5) cells, P0.05], which was significantly attenuated by treatment with Irb. AngII significantly increased the total protein content in HK-2 cells [control: (0.169 +/- 0.011) mg/10(5) cells vs AngII group: (0.202 +/- 0.010) mg/10(5) cells, P0.05], which was also markedly inhibited by cotreatment with Irb (P0.01). Scanning electron microscopy showed that AngII induced an increase in average physical cell size, which was significantly inhibited by Irb [control: (11.92 +/- 1.62) microm; AngII group: (20.63 +/- 3.83) micro m; AngII + Irb group: (13.59 +/- 3.15) micro m; P0.01 vs control, respectively]. Furthermore, flow cytometry revealed that AngII arrested cells in the G(0)-G(1) phase, which was significantly reversed by treatment with Irb [G(0)-G(1) cells in AngII group: (76.09 +/- 1.82)%, in AngII + Irb group: (67.00 +/- 2.52)%, P0.05].Irb can inhibit AngII-induced hypertrophy in HK-2 cells.
- Published
- 2004
42. Esophagogastric variceal bleeding in cirrhotic portal hypertension: consensus on prevention and management (2008).
- Author
-
Cheng LF, Jia JD, Xu XY, Linghu EQ, Liu YL, Leng XS, and Wang MQ
- Subjects
- Endoscopy, Digestive System, Esophageal and Gastric Varices complications, Esophageal and Gastric Varices therapy, Fibrosis complications, Gastrointestinal Hemorrhage etiology, Gastrointestinal Hemorrhage therapy, Humans, Hypertension, Portal etiology, Hypertension, Portal therapy, Esophageal and Gastric Varices drug therapy, Esophageal and Gastric Varices surgery, Gastrointestinal Hemorrhage drug therapy, Gastrointestinal Hemorrhage surgery, Hypertension, Portal drug therapy, Hypertension, Portal surgery
- Published
- 2009
43. Hematopoietic-supportive effect of (2S, 3R)-ent-catechin on marrow-depressed mice.
- Author
-
Chen YH, Wang DX, Liu P, Chen RY, Chen ML, Cheng LF, Yin JF, and Chen GY
- Subjects
- Animals, Female, Gene Expression Regulation drug effects, Granulocyte-Macrophage Colony-Stimulating Factor blood, Granulocyte-Macrophage Colony-Stimulating Factor genetics, Interleukin-6 blood, Interleukin-6 genetics, Male, Mice, RNA, Messenger analysis, Bone Marrow drug effects, Catechin pharmacology, Fabaceae chemistry, Hematopoiesis drug effects, Plants, Medicinal chemistry
- Published
- 2005
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