1. Study of van Gieson's picrofuchsin staining on second-harmonic generation in type I collagen
- Author
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Hanping Liu, Xiaoyuan Deng, Lingling Zhao, Junle Qu, and Zhengfei Zhuang
- Subjects
animal structures ,Chemistry ,business.industry ,Second-harmonic generation ,Nanotechnology ,Atomic and Molecular Physics, and Optics ,Electronic, Optical and Magnetic Materials ,Staining ,Hydroxyproline ,chemistry.chemical_compound ,Optics ,Nuclear magnetic resonance ,Glycine ,Microscopy ,Van Gieson's stain ,Electrical and Electronic Engineering ,business ,Biological imaging ,Type I collagen - Abstract
Second-harmonic generation (SHG) microscopy is a recently developed nonlinear optical imaging modality for imaging tissue structures with submicron resolution and is a potent tool for visualizing pathological effects of diseases. In this letter, we present our investigation on the influence of van Gieson's (VG) alcoholic picrofuchsin staining on SHG in type I collagen (from tendon-rich C57BL/6). Multi-channel imaging and spectra analysis show that the strong SHG signal produced in fresh collagen type I fiber has been greatly suppressed after VG staining, which indicates that staining may induce the structural or characteristic changes of SHG-dependent crystal formed by collagen constituents, such as glycine, proline, and hydroxyproline.
- Published
- 2008
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