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The expression and regulation of metalloproteinases-2, -9 (MMP-2, -9) and their tissue inhibitors TIMP-1,-2, -3 mRNA were studied in this experiment. In the PMSG-hCG primed pseudopregnant rat, MMP-2, -9 mRNA levels were the highest at Day 1, decreased from Day 4, and reached the minimal level at Day 8, then increased at Day 14; no significant changes were observed in TIMP-2 mRNA expression from Day 1 to Day 14; TIMP-3 mRNA expression was the lowest at Day 1, increased from Day 4, reached the maximal level at Day 8, and persisted to Day 14. TNF-α could significantly increase the expression of MMP-2, -9 and TIMP-1 mRNA in thein vitro perfused pseudopregnant CL, and decrease the expression of TIMP-3 mRNA, but had no effect on TIMP-2 mRNA expression. The results indicate that MMP-2, -9 and TIMP-1, -2, -3 might be involved in the regulation of CL function and maintenance of CL structure via their coordinated gene expression. TNF-a could inhibit luteal regression via increasing MMP-2, -9 and TIMP-1 mRNA in thein vitro perfused pseudopregnant ovary. [ABSTRACT FROM AUTHOR]

Discusses the expression and regulation of metalloproteinases-2, -9 (MMP-2, -9) and their tissue inhibitors TIMP-1, -2, -3 mRNA. Observation of MMP-2, -9 mRNA levels in the PMSGhCG primed pseudopregnant rat; Indication there were no significant changes in TIMP-2 mRNA expression from Day 1 to Day 14; TIMP-3 mRNA expression was the lowest at Day 1; Suggestion that TNF-α can increase the expression of MMP-2, -9 and TIMP-1 mRNA in the in vitro perfused pseudopregnant CL, decrease the expression of TIMP-3 mRNA, and have no effect on TIMP-2 mRNA expression; Conclusion that MMP-2, -9 and TIMP-1, -2, -3 could be involved in the regulation of CL function and the maintenance of CL structure through their coordinated gene expression; Assertion that TNF-α could inhibit luteal regression in the in vitro perfused pseudopregnant ovary.

Presents information on a study which investigated the expression of gelatinases and tissue inhibitors of metalloproteinases (TIMP) in the rhesus monkey corpus luteum (CL) in luteal and pregnancy stages. Role of matrix metalloproteinases and TIMP in the development of CL; Methodology; Results and discussion.