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Chiral resolution of binaphthylamine is often a toilful conundrum in the field of analytical chemistry and biomedicine. The work puts forward a selective, sensitive, and miniaturized analytical method based on molecularly imprinted polymers (MIPs) as adsorbent for miniaturized tip solid‐phase extraction (MTSPE) in the separation of binaphthylamine enantiomer. This method combines the advantages of MIPs (high selectivity), MTSPE (low consumption), and high‐performance liquid chromatography (HPLC, high sensitivity). A simple synthesis methodology of MIP (P2) was conducted through bulk polymerization with (S)‐(−)‐1,1′‐binaphthyl‐2,2′‐diamine (S‐DABN) as template together with methacrylic acid monomer, and ethylene glycol dimethacrylate as cross‐linker in proper porogen, realizing a selective recognition and efficient enrichment for S‐DABN. The method exhibited appreciable linearity (0.06–1.00 mg ml−1), low quantification limit (0.056 mg ml−1), good absolute recoveries (45.70%–69.29%), and high precision (relative standard deviations ≤ 3.54%), along with low consumption (0.50 ml sample solution and 25.0 mg adsorbent). Based on the density functional theory, computational simulation was used to make a preliminary prediction for rational design of MIPs and gave a reasonable elaboration involving the potential mechanism of templates interacting with functional monomers. The adsorption kinetics and thermodynamics were investigated to evaluate the recombination process of substrates. In addition, the selectivity of MIPs for S‐DABN was obtained by MIP‐MTSPE coupled with HPLC, which supports the feasibility of this convenient design process. The proposed method was employed for selective extraction of S‐DABN and exhibited promising potential in the application of chiral analysis. [ABSTRACT FROM AUTHOR]