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This paper describes an HPLC method for the determination of cefuroxime in human plasma. The method uses solid phase extraction (SPE) and has acceptable sensitivity, precision and accuracy. The limit of quantification in plasma samples is 0.1 μg mL−1. Calibration curves were linear within 0.1–20 μg mL−1, with mean correlation coefficient of 0.9982. Mean inter day precision and accuracy were 7.8% and 6.4%, respectively. The method was applied to determine cefuroxime levels in patients receiving cefuroxime, 3 time per day.

This paper describes an HPLC method for the determination of meropenem in human plasma. The method uses solid phase extraction (SPE) of the samples and has good sensitivity, precision and accuracy. The limit of quantification in plasma samples is 0.02 μg mL−1. Calibration curves were linear over a large dynamic range, namely within 0.02–50 μg mL−1. The method was applied to the determination of meropenem levels in patients receiving meropenem, as a single dose or at steady state.

A simple, low-cost, sensitive and selective HPLC method was developed for the determination of phenazopyridine in human plasma. The method employs UV detection of phenazopyridine and of the internal Standard at 2 different wavelengths. Calibration curves were linear over a large dynamic range, i.e., within 0.05–10.0 μg mL−1 with limit of quantification of 0.05 μg mL−1, and a limit of detection of 0.01 μg mL−1.