1. Dexamethasone Influences Human Clock Gene Expression in Bronchial Epithelium and Peripheral Blood Mononuclear Cells in vitro.
- Author
-
Burioka, Naoto, Takata, Miyako, Okano, Yoko, Ohdo, Shigehiro, Fukuoka, Yasushi, Miyata, Masanori, Takane, Hiroshi, Endo, Masahiro, Suyama, Hisashi, and Shimizu, Eiji
- Subjects
- *
BIOLOGICAL rhythms , *CHRONOBIOLOGY , *CIRCADIAN rhythms , *GENE expression , *CELLS , *EPITHELIUM - Abstract
We determined whether human peripheral blood mononuclear cells (PBMCs) could be used to analyze clock genes by studying their mRNA expressions in human bronchial epithelium (BEAS‐2B) and PBMCs following stimulation by the glucocorticoid homologue dexamethasone (DEX) in vitro. PBMCs were obtained at 10:00 h from two diurnally active (∼⃒07:00 to 23:00 h) healthy volunteers and were evaluated for h Per1 mRNA expression following DEX stimulation in vitro using real time‐PCR analysis. DEX stimulation of human BEAS‐2B cells and PBMCs in vitro led to a remarkable increase of h Per1 mRNA. The glucocorticoid rapidly affected the expression of h Per1 mRNA in PBMCs, suggesting that human PBMCs may be a useful surrogate marker for the investigation of drug effects on clock genes. [ABSTRACT FROM AUTHOR]
- Published
- 2005
- Full Text
- View/download PDF