1. Overexpression and clinical significance of carcinoembryonic antigen-related cell adhesion molecule 6 in colorectal cancer
- Author
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Chang Nam Kim, Young Il Yeom, Kyo Hyun Kim, Jong-Tae Kim, Young-Ha Lee, Myoung-Soo Nam, In Seong Choe, Seon-Jin Lee, Min Ah Kang, Yun Hee Kang, Hee Gu Lee, Seon-Young Kim, Jong-Wan Kim, Joo Heon Kim, and Kwang Soo Kim
- Subjects
Adult ,Male ,Pathology ,medicine.medical_specialty ,Colorectal cancer ,Clinical Biochemistry ,Adenocarcinoma ,GPI-Linked Proteins ,Biochemistry ,Carcinoembryonic antigen ,Antigens, CD ,Cell Line, Tumor ,medicine ,Biomarkers, Tumor ,Humans ,Anoikis ,Neoplasm Invasiveness ,Gene Silencing ,RNA, Small Interfering ,Tumor marker ,Aged ,Oligonucleotide Array Sequence Analysis ,Tissue microarray ,biology ,Cell adhesion molecule ,Reverse Transcriptase Polymerase Chain Reaction ,Biochemistry (medical) ,General Medicine ,Middle Aged ,medicine.disease ,Prognosis ,Survival Analysis ,Gene Expression Regulation, Neoplastic ,Cancer cell ,biology.protein ,Immunohistochemistry ,Female ,Colorectal Neoplasms ,Cell Adhesion Molecules - Abstract
Carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6) inhibits anoikis and affects the malignant phenotype of cancer cells. In this study, we analyzed CEACAM6 as a gene that is highly upregulated in colon cancer tissues, and examined the assertion that CEACAM6 might be a suitable candidate tumor marker for the diagnosis of colon cancer.CEACAM6 gene expression in human colon tissues was performed by tissue microarray and analyzed using RT-PCR (each of normal and tumor tissue, n=40) and immunohistochemical and clinicopathological (colon cancer patients, n=143) analyses.CEACAM6 transcriptional and translational levels were significantly upregulated in human tumor tissues compared to non-tumor regions, and clinicopathological analysis revealed a significant correlation between CEACAM6 protein expression and Dukes' stage (p0.001). High expression levels of CEACAM6 were significantly associated with lower overall survival (p0.001) and shorter recurrence-free survival (p0.001). We demonstrated that knockdown of CEACAM6 with CEACAM6-specific small interfering RNA in colorectal cancer cells attenuated invasivity (35%); conversely, the overexpression of CEACAM6 increased invasiveness.CEACAM6 is significantly upregulated in colon cancer tissues and is closely associated with poor prognosis, indicating that CEACAM6 might be used as a tumor biomarker and a potential therapeutic target for colon cancer.
- Published
- 2012