1. Immunodiagnosis of the primary brain tumor (glioma) by the endogenous lectin
- Author
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Ramdhan Majhi, Pranab S. Basu, Sandip K. Batabyal, Samarendranath Ghosh, and Bhaskar Ghosh
- Subjects
Male ,Pathology ,medicine.medical_specialty ,Clinical Biochemistry ,Cross Reactions ,Biochemistry ,Carcinoembryonic antigen ,Cerebrospinal fluid ,Antigen ,Reference Values ,Glioma ,Lectins ,medicine ,Humans ,Cerebrospinal Fluid ,Antiserum ,biology ,Brain Neoplasms ,Biochemistry (medical) ,Lectin ,General Medicine ,Middle Aged ,medicine.disease ,Molecular biology ,Body Fluids ,Carcinoembryonic Antigen ,Case-Control Studies ,biology.protein ,Female ,alpha-Fetoproteins ,Antibody ,Alpha-fetoprotein - Abstract
Background : A sugar-binding protein, lectin, was identified in the cystic fluid of brain tumor (glioma). Methods : The protein was purified by high performance liquid chromatography. Results : The molecular weight of the lectin was 29 kDa. The activity of the purified protein was inhibited strongly by p -nitro-β- d -galactose and asialofetuin. The lectin activity was not Ca 2+ - or Mg 2+ -dependent. The antiserum of this pure lectin specifically cross-reacted in the cerebrospinal fluid (CSF) of glioma tumor patients. The same antibody had no reaction with CSF of other neurological patients, normal rat brain tissue extract, oncofetal antigens such as alpha fetoprotein (AFP), carcinoembryonic antigen (CEA), plant lectins, and other body fluids like plural and ascites. Conclusions : This antilectin antibody may be useful in the evaluation of patients with primary brain tumor (glioma).
- Published
- 2002