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Start Over Author "Mendonça MG" Journal clinical and diagnostic laboratory immunology

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1. Identification of potentially diagnostic Leishmania braziliensis antigens in human cutaneous leishmaniasis by immunoblot analysis.

Author

Brito ME, Mendonça MG, Gomes YM, Jardim ML, and Abath FG

Subjects
Adolescent, Adult, Aged, Aged, 80 and over, Animals, Child, Child, Preschool, Female, Humans, Immunoblotting methods, Leishmaniasis, Cutaneous blood, Leishmaniasis, Cutaneous diagnosis, Male, Middle Aged, Sensitivity and Specificity, Antibodies, Protozoan blood, Antigens, Protozoan immunology, Leishmania braziliensis immunology, Leishmaniasis, Cutaneous immunology
Abstract

The antibody response in patients with American cutaneous leishmaniasis was analyzed by immunoblotting with soluble and insoluble antigens of Leishmania braziliensis. The recognition of the 27- and/or 30-kDa soluble antigens was considered relevant for the diagnosis of cutaneous leishmaniasis. Immunoblotting was found to be significantly more sensitive and specific than indirect immunofluorescence and enzyme-linked immunosorbent assay.

Published
2000
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