1. [Untitled]
- Author
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Shinichi Nakano, Etsuo Yoshida, Kiyotaka Yokogami, Tetsuro Sameshima, Ryouichi Hamasuna, Takuzou Moriyama, Hiroaki Kataoka, Tsutomu Iseda, Masashi Koono, and Shinichiro Wakisaka
- Subjects
Urokinase ,Cancer Research ,Chemistry ,General Medicine ,medicine.disease ,Urokinase receptor ,Oncology ,Downregulation and upregulation ,Glioma ,Immunology ,medicine ,Cancer research ,Hepatocyte growth factor ,Autocrine signalling ,Receptor ,Plasminogen activator ,medicine.drug - Abstract
Several lines of evidence indicate that hepatocyte growth factor/scatter factor (HGF/SF) and its receptor, c-Met, may play an important role in progression of human glioma. In this study, effects of HGF/SF on urokinase- type plasminogen activator (uPA)-mediated proteolysis network were examined in c-Met-positive human glioma cell lines. Treatment of the glioma cells with various concentrations of HGF/SF resulted in an enhanced secretion of uPA proteins accompanying increased transcription of uPA mRNA in a dose dependent fashion. The levels of uPA receptor (uPAR) mRNAs were also elevated simultaneously upon HGF/SF stimulation, and the cell-surface associated uPA activity was also elevated by the treatment. Since concomitant expression of HGF and its receptor c-Met are frequently observed in malignant gliomas, these results suggest that HGF/SF participates in invasive process of malignant glioma cells not only by its motility-stimulating activity but also through enhanced degradation of the extracellular matrix induced by autocrine activation of uPA proteolysis network.
- Published
- 1999
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