Search

Your search keyword '"Giugliani, Roberto"' showing total 13 results
Start Over Author "Giugliani, Roberto" Journal clinical biochemistry
13 results on '"Giugliani, Roberto"'

13. Comparison of the measurement of lysosomal hydrolase activity in mycoplasma-contaminated and non-contaminated human fibroblast cultures treated with mycoplasma removal agent.

Author

Souza FT, Souza Sostruznik L, Casagrande Scolari R, Maciel de Castro KJ, Giugliani R, and Coelho JC

Subjects
Cells, Cultured, Enzyme Activation drug effects, Fibroblasts enzymology, Fibroblasts microbiology, Glucosidases metabolism, Glucuronidase metabolism, Hexosaminidases metabolism, Humans, Mycoplasma growth & development, Quinolones pharmacology, beta-Galactosidase metabolism, Fibroblasts drug effects, Hydrolases metabolism, Lysosomes enzymology, Mycoplasma drug effects
Abstract

Objectives: To investigate the effect of both mycoplasma contamination and of its remover (MRA), through human fibroblasts culture over the activity of some lysosomal hydrolases., Design and Methods: Activity was measured in contaminated fibroblasts before and after the addition of MRA. Results were compared with the enzymatic activity in control fibroblasts with and without MRA., Results: Only beta-glucosidase showed no significant alteration in the presence of either mycoplasma or MRA. Total hexosaminidase and beta-galactosidase underwent significant interference in the presence of the mycoplasma and the MRA. The % of hexosaminidase A and arylsulphatase A altered their activity only in the presence of MRA. Beta-glucuronidase changed its activity only in the presence of mycoplasma., Conclusions: The fibroblast enzymes behaved differently in the presence of MRA and/or mycoplasma, demonstrating the sensitivity of these hydrolases. Our work suggests that mycoplasma and MRA alter the activity of some lysosomal hydrolases.

Published
2007
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results