1. Lateral flow assay for simultaneous detection of cellular- and humoral immune responses.
- Author
-
Corstjens PL, de Dood CJ, van der Ploeg-van Schip JJ, Wiesmeijer KC, Riuttamäki T, van Meijgaarden KE, Spencer JS, Tanke HJ, Ottenhoff TH, and Geluk A
- Subjects
- Antigens, Bacterial analysis, Biomarkers blood, Buffers, Cytokines metabolism, Enzyme-Linked Immunosorbent Assay, Glycolipids analysis, Humans, Immunity, Humoral, Interferon-gamma metabolism, Leprosy blood, Mycobacterium Infections diagnosis, Mycobacterium Infections immunology, Reference Values, Sensitivity and Specificity, Biological Assay methods, Cytokines blood, Interferon-gamma analysis, Interleukin-10 blood, Leprosy diagnosis, Leprosy immunology
- Abstract
Objective: The development of a cytokine detection assay suitable for detection of multiple biomarkers for improved diagnosis of mycobacterial diseases., Design and Methods: A lateral flow (LF) assay to detect IL-10 was developed utilizing the up-converting phosphor (UCP) reporter-technology. The assay was evaluated using blood samples of leprosy patients. Multiplex applications were explored targeting: 1) IL-10 and IFN-γ in assay buffer; 2) IL-10 and anti-phenolic glycolipid (PGL-I) antibodies in serum from leprosy patients., Results: Detection of IL-10 below the targeted level of 100pg/mL in serum was shown. Comparison with ELISA showed a quantitative correlation with R(2) value of 0.92. Multiplexing of cytokines and simultaneous detection of cytokine and antibody was demonstrated., Conclusions: The UCP-LF IL-10 assay is a user-friendly, rapid alternative for IL-10 ELISAs, suitable for multiplex detection of different cytokines and can be merged with antibody-detection assays to simultaneously detect cellular- and humoral immunity., (Copyright © 2011 Elsevier B.V. All rights reserved.)
- Published
- 2011
- Full Text
- View/download PDF