1. Sodium dodecyl sulfate-agarose gel electrophoresis of urinary proteins: application to multiple myeloma.
- Author
-
Le Bricon T, Erlich D, Bengoufa D, Dussaucy M, Garnier JP, and Bousquet B
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Electrophoresis, Agar Gel methods, Female, Humans, Immunoelectrophoresis, Immunoglobulin Light Chains urine, Male, Middle Aged, Multiple Myeloma complications, Nephelometry and Turbidimetry methods, Proteinuria complications, Reproducibility of Results, Sensitivity and Specificity, Sodium Dodecyl Sulfate, Bence Jones Protein urine, Multiple Myeloma urine, Proteinuria urine
- Abstract
We evaluated a new sodium dodecyl sulfate-agarose gel electrophoresis (SDS-AGE) for urinary protein analysis in patients with multiple myeloma (MM; n = 47; ages, 62 +/- 2 years, mean +/- SE). Abnormal proteinuria (mean = 1872 +/- 360 mg/24 h) was present in 95% of the samples; 75% of the patients had some sign of renal dysfunction (glomerular and/or tubular) according to their SDS-AGE pattern. A band suggesting Bence Jones proteinuria (BJP) was detected in 40 vs 33 specimens by routine AGE. Immunofixation identified BJP in 38 patients; the calculated sensitivity of SDS-AGE for BJP was 97%. Excellent correlation (P <0.0001) was obtained with routine AGE (r = 0.994) and immunonephelometry (r = 0.963) for light chain quantification. SDS-AGE allows easy evaluation of renal dysfunction and shows high sensitivity for BJP detection. In a specialized laboratory, it is useful for following the progress of MM patients through the semiquantification of BJP.
- Published
- 1998