1. In Black Africans with rheumatoid arthritis, ACPA recognize citrullinated fibrinogen and the derived peptides α36-50Cit38,42 and β60-74Cit60,72,74, like in Caucasians
- Author
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Sylvette Bas, Leonor Nogueira, Madeleine Singwe-Ngandeu, Guy Serre, Cem Gabay, Martin Cornillet, Sebastien Viatte, Unité différenciation épidermique et auto-immunité rhumatoïde (UDEAR), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM), CHU Toulouse [Toulouse], University of Yaoundé [Cameroun], Manchester Academic Health Science Centre (MAHSC), University of Manchester [Manchester], Hôpital Universitaire de Genève, and University of Geneva [Switzerland]
- Subjects
Arthritis, Rheumatoid/diagnosis/genetics/immunology ,MESH: Amino Acid Sequence ,ddc:616.07 ,MESH: Africa ,Fibrinogen ,Epitope ,Arthritis, Rheumatoid ,Epitopes ,Anti-citrullinated protein autoantibodies (ACPA) ,MESH: Arthritis, Rheumatoid / genetics ,MESH: Arthritis, Rheumatoid / immunology ,[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases ,MESH: Epitopes / genetics ,Peptides/genetics/immunology ,Immunology and Allergy ,Medicine ,Citrullinated fibrin ,MESH: HLA-DRB1 Chains ,African Continental Ancestry Group ,ddc:616 ,biology ,MESH: European Continental Ancestry Group ,MESH: Biomarkers / analysis ,3. Good health ,MESH: Epitopes / immunology ,Rheumatoid arthritis ,Antibody ,Autoantibodies/immunology ,medicine.drug ,Black african ,MESH: Peptides / genetics ,Epitopes/genetics/immunology ,European Continental Ancestry Group ,Immunology ,MESH: Autoantibodies / immunology ,Black People ,MESH: Peptides / immunology ,White People ,Fibrin ,MESH: Citrulline / immunology ,MESH: Fibrinogen / immunology ,Humans ,Amino Acid Sequence ,Allele ,Autoantibodies ,MESH: Arthritis, Rheumatoid / diagnosis ,MESH: Humans ,business.industry ,Black Africans ,Autoantibody ,Fibrinogen/immunology ,medicine.disease ,B-cell epitopes ,Africa ,Citrulline/immunology ,biology.protein ,Citrulline ,Biological Markers/analysis ,MESH: African Continental Ancestry Group ,Peptides ,business ,Biomarkers ,HLA-DRB1 Chains - Abstract
International audience; Well documented in Caucasians and Asians, the diagnostic value of anti-CCP2 antibodies has been confirmed in Black African populations. However, autoantibodies to other citrullinated peptides/proteins and their fine specificities have not yet been studied. Here, we show that in Cameroonian patients, anti-citrullinated fibrinogen autoantibodies (AhFibA) are sensitive (73%) diagnostic markers for RA. We also determine that autoantibodies directed to α36-50Cit38,42 or β60-74Cit60,72,74 peptides which bear the immunodominant epitopes of citrullinated fibrin, are present in similar proportions in Black Africans and Caucasians with 25/56 (45%) and 41/56 (73%) positive RA-sera in Cameroonians, respectively. They also account for almost all the AhFibA reactivities since 38/41 (93%) AhFibA-positive sera contain anti-α36-50Cit38,42 and/or anti-β60-74Cit60,72,74 autoantibodies. Finally, HLA-DRB1 SE alleles were associated with higher titres of AhFibA and anti-β60-74Cit60,72,74 autoantibodies. In the genetic and environmental backgrounds of Black Africans, AhFibA are a hallmark of RA like in Caucasians, moreover they recognize the same fibrin epitopes.
- Published
- 2014
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