1. Alteration of the cytokine signature by various TLR ligands in different T cell populations in MOG37-50 and MOG35-55-induced EAE in C57BL/6 mice.
- Author
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Steckner C, Weber A, Mausberg AK, Heininger M, Opdenhövel F, Kieseier BC, Hartung HP, and Hofstetter HH
- Subjects
- Animals, CD4-Positive T-Lymphocytes drug effects, CD4-Positive T-Lymphocytes immunology, CD4-Positive T-Lymphocytes metabolism, CD8-Positive T-Lymphocytes drug effects, CD8-Positive T-Lymphocytes immunology, CD8-Positive T-Lymphocytes metabolism, Cells, Cultured, Central Nervous System immunology, Central Nervous System metabolism, Cytokines genetics, Cytokines metabolism, Encephalomyelitis, Autoimmune, Experimental metabolism, Enzyme-Linked Immunospot Assay, Female, Flow Cytometry, Gene Expression drug effects, Gene Expression immunology, Guanosine analogs & derivatives, Guanosine immunology, Guanosine pharmacology, Interferon-gamma immunology, Interferon-gamma metabolism, Interleukin-17 genetics, Interleukin-17 immunology, Interleukin-17 metabolism, Ligands, Lipopolysaccharides immunology, Lipopolysaccharides pharmacology, Mice, Inbred C57BL, Peptide Fragments immunology, Reverse Transcriptase Polymerase Chain Reaction, T-Lymphocyte Subsets drug effects, T-Lymphocyte Subsets metabolism, Time Factors, Toll-Like Receptors agonists, Toll-Like Receptors metabolism, Cytokines immunology, Encephalomyelitis, Autoimmune, Experimental immunology, Myelin-Oligodendrocyte Glycoprotein immunology, T-Lymphocyte Subsets immunology, Toll-Like Receptors immunology
- Abstract
Interleukin 17 (IL-17), produced by T cells, plays an important role in Multiple Sclerosis (MS) and its animal model, Experimental Autoimmune Encephalomyelitis (EAE). In contrast to IL-17-producing CD4+ T cells, the contribution of IL-17-producing CD8+ T cells (Tc17) in CNS autoimmunity has been investigated less intensively. Here we investigate the role of TC17 in EAE. We compare different T cell populations and their cytokine pattern in the MOG35-55- and MOG37-50-induced EAE. We detected a similar cytokine phenotype for both EAE models in the autoimmune process assessed at different stages. Regarding the migratory activity, an involvement of IL-17 and IFN-γ in disease onset was suggested. Furthermore, we show that PAMPs have the ability to drive autoimmune process. To modify the cytokine pattern of different T cell populations, a combination of distinct factors is required (the activation of MyD88 or Syk, the genetic background, the presence of APCs and CD4+ T cells)., (Copyright © 2016 Elsevier Inc. All rights reserved.)
- Published
- 2016
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