1. Effects of arginine supplementation on mucosal immunity in rats with septic peritonitis
- Author
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Wan Chun Chiu, Yun Ni Lai, Sung Ling Yeh, Huey Fang Shang, and Yi Yun Wang
- Subjects
Immunoglobulin A ,Male ,medicine.medical_specialty ,Lymphocyte ,T-Lymphocytes ,Peritonitis ,Critical Care and Intensive Care Medicine ,Arginine ,Enteral administration ,Sepsis ,Peyer's Patches ,Random Allocation ,Immunity ,Internal medicine ,Medicine ,Animals ,Lymphocyte Count ,Rats, Wistar ,Immunity, Mucosal ,Nutrition and Dietetics ,biology ,business.industry ,medicine.disease ,Lymphocyte Subsets ,Rats ,Endocrinology ,medicine.anatomical_structure ,Parenteral nutrition ,Immunology ,Dietary Supplements ,biology.protein ,Cytokines ,Cytokine secretion ,Parenteral Nutrition, Total ,business - Abstract
Background : Supplemental Arginine (Arg) has been demonstrated to improve the immunologic response and reduce mortality in rodents with sepsis. However, the effects of Arg on gut-associated lymphoid tissue function after infection and sepsis are not clear. The aim of this study was to study the effect of Arg-supplemented diets before and Arg-enriched total parenteral nutrition (TPN) after sepsis or both on the intestinal immunity of rats with septic peritonitis. Methods : Rats were assigned to four groups. Groups 1 and 2 were fed a semipurified diet, while in the diets of groups 3 and 4, part of the casein was replaced with Arg. After feeding the experimental diets for 10 days, sepsis was induced by cecal ligation and puncture (CLP); at the same time, the internal jugular vein was cannulated. All rats were maintained on TPN for 3 days. Groups 1 and 3 were infused with conventional TPN, while groups 2 and 4 were given a TPN solution supplemented with Arg, which replaced 10% of the total amino acids. All rats were sacrificed 3 days after CLP. Intestinal immunoglobin (Ig) A levels, total lymphocyte yields, and lymphocyte subpopulations in Peyer's patches were analyzed. In vitro cytokine secretion by splenocytes and Peyer's patch lymphocytes were also measured. Results : Total lymphocyte yields in Peyer's patches, and small intestinal immunoglobulin A (IgA) secretion in group 4 were significantly higher than the groups 1 and 2. No differences were observed between groups 3 and 4. There were no differences in the interleukin (IL)-2 and interferon- γ levels among all groups when splenocytes were stimulated with mitogen. However, in vitro splenocyte IL-10 production in group 4 was significantly higher than those of groups 1 and 2, and had no difference from group 3. There were no differences in the ratios of B and T lymphocyte subpopulations in Peyer's patches among all groups. Conclusions : Enteral Arg supplementation before sepsis tended to enhance total lymphocyte yields in Peyer's patches and intestinal IgA secretion. Arg administered both before and after CLP had a synergistic effect on improving intestinal immunity, possibly by enhancing systemic IL-10 secretion. However, intravenous Arg administration after CLP had no favorable effects on mucosal immunity in rats with septic peritonitis.
- Published
- 2003