1. WT1-specific CTL cells of recipient origin may exist in the peripheral blood of patients achieving full donor chimerism soon after nonmyeloablative transplantation
- Author
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Li, Wei, Hongli, Zuo, Xuedong, Sun, Tieqiang, Liu, Mei, Guo, Guangxian, Liu, Qiyun, Sun, Jianhui, Qiao, Danhong, Wang, Changlin, Yu, Kaixun, Hu, Zheng, Dong, and Huisheng, Ai
- Subjects
Adult ,Male ,Transplantation Chimera ,Leukemia ,Sex Chromosomes ,Adolescent ,Hematopoietic Stem Cell Transplantation ,Graft vs Host Disease ,DNA, Neoplasm ,CD8-Positive T-Lymphocytes ,Middle Aged ,Prognosis ,Real-Time Polymerase Chain Reaction ,Tissue Donors ,Young Adult ,HLA-A2 Antigen ,Humans ,Transplantation, Homologous ,Female ,Child ,WT1 Proteins ,In Situ Hybridization, Fluorescence ,T-Lymphocytes, Cytotoxic - Abstract
This study was performed to assay whether leukemia-associated antigen (LAA)-specific CTLs of recipient origin existed in the blood of patients who achieved full donor chimerism (FDC) soon after nonmyeloablative transplantation (NST). In 15 patients who received haplo-identical NST, WT1(+) CD8(+) CTLs were detected with WT1/HLA-A*0201 pentamer, and the donor-recipient chimerism levels were analyzed by three methods. Results showed that WT1(+) CD8(+) CTLs could be detected in patients with HLA-A*0201 expressing only in recipient, and cells of recipient origin existed in the blood of patients who achieved FDC, which suggested that LAA-specific CTLs of recipient origin may exist in patients achieving FDC soon after NST.
- Published
- 2011