Glycation is a non-enzymatic biochemical reaction between reducing sugars and amino acids, causing the crosslinking and rearrangement of glycated proteins, leading to irreversible formation of Advanced Glycation End products (AGEs). Glycation is an activity that occurred both endogenously in our body and exposed to it through our diet as well, which contributed to the pool of AGEs and their pathology. AGEs played important role in various health conditions including and not limited to, hyperglycemia, inflammation, Alzheimer’s disease, cardiovascular health and, ageing itself. While the formation of AGEs is irreversible, its formation can be inhibited or slowed down by natural products, which have anti-glycation activity. In this study, we explored the antiglycation activity of commercial herbal extracts of Artocarpus lakoocha and Pterocarpus marsupium using a competitive fluorescence assay. Both Artocarpus lakoocha and Pterocarpus marsupium extracts show highest anti-glycation activity (84.6–100%) after background correction, in a range of 10 to 100 mg/ml.