1. Construction of ordered multienzyme systems using multifunctional polymer brush-grafted magnetic nanoparticles as scaffolds.
- Author
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Chen, Zhiwu, Liang, Xingxing, Yang, Rui, Yang, Miao, Tan, Tianwei, and Cao, Hui
- Subjects
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MULTIENZYME complexes , *MAGNETIC nanoparticles , *POLYMERS , *GLUCOSE oxidase , *ACRYLATES , *HORSERADISH peroxidase , *CATALYTIC activity - Abstract
A block copolymer brush based on functional monomers (N -succinimidyl acrylate and N -adamantylacrylamide) successfully grew on the surface of Fe 3 O 4 @SiO 2 nanoparticles by surface-initiated reversible addition-fragmentation chain transfer polymerization (SI-RAFT). According to the multiple recognition mechanisms of this material, horseradish peroxidase (HRP) and β-cyclodextrin-modified glucose oxidase (GOx-CD) were constructed into a multienzyme system with an ordered distribution for the cascade reaction, where multifunctional polymer brush-grafted magnetic nanoparticles were used as the scaffolds. The catalytic activity of GOx-CD was enhanced compared with free GOx. In addition to being easily recycled, this multienzyme system exhibited an outstanding conditional stability compared with the free enzyme system. After being reused 8 times, the activity of multienzyme system retained 67.21% of the initial catalytic activity. This strategy provides a novel idea for reusable ordered enzyme immobilization. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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