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Pickering emulsion have been widely used to encapsulate bioactives with the aim of preventing degradation, improving bioavailability and achieving the effect of slow release. Curcumin (Cur), as a bioactive compound, was famous as its excellent beneficial human health functions. However, the low solubility and susceptibility to environmental influences limit the application of Cur. In our previous study, the covalent nanoparticles (sugarcane leaf polyphenols-zein, SGLpZ) with excellent antioxidant capacity were successfully fabricated. In addition, the pickering emulsion stabilized using SGLpZ with significance storage stability and antioxidant properties, which inspired us for Cur delivery application. Therefore, in this study, SZP (pickering emulsion stabilized by SGLpZ) were prepared and were firstly used to delivery Cur evaluation. Results exhibited that the mechanism of excellent stabilization of SZP may be due to the wide range of O/W phase interfaces with SGLpZ, which could be further used as a delivery system for Cur. An in-vitro simulated digestion suggested that SZP provided better protection to Cur under three different conditions (different Na+ concentration, temperatures and UV exposure times), compared to ZZP (pickering emulsion stabilized by zein nanoparticles alone). SZP was able to achieve a slow release of Cur as well as the high bioaccessibility of Cur during the in-vitro simulated digestion. Finally, Cur, Cur-ZZP and Cur-SZP inhibited respectively by 33.20±1.00%, 53.26±0.94% and 71.59±0.14% (p ＜0.05) at the Cur concentration of 20 μg/mL in the in-vitro inhibition of AAPH-induced erythrocyte hemolysis assay. The study indicated that the pickering emulsion stabilized by SGLpZ was a reliable Cur delivery system and provided a novel idea of food material application for bioacitive substances delivery. [Display omitted] • A green-sustainable pickering emulsion stabilized by Sugarcane leaf polyphenols-Zein nanoparticles was firstly prepared. • Cur-SZP for better results compared with curcumin and Cur-ZZP in three different external environments. • Cur-SZP achieved slow release and improved bioavailability of curcumin in in-vitro digestion. • The inhibitory effect of Cur-SZP on hemolysis of red blood cells was studied. [ABSTRACT FROM AUTHOR]

A novel strategy was devised to synthesize robust nanocarriers by conjugating fish collagen peptides (FCP) with Ampelopsis grossedentata polysaccharide (AGP), culminating in the creation of FCP-AGP nanoparticles (FCAGPs) for advanced drug delivery systems. The innovative approach entailed the systematic investigation of particle size, zeta potential, and surface hydrophobicity alterations in FCAGPs upon varying AGP concentrations. The optimized FCAGP-4 nanoparticles, boasting a precisely engineered AGP content of 2.0 % (w/v) and an average particle diameter finely tuned to 353.2 ± 10.5 nm, demonstrated outstanding characteristics in dispersibility, stability, and emulsifying capacity. Furthermore, the water contact angle of FCAGP nanoparticles notably increased from 46.3 ± 2.8° to 70.2 ± 5.6°, indicating enhanced surface hydrophobicity. The mechanism by which FCAGP nanoparticles were attached to the surface of oil droplets to stabilize the emulsions was elucidated by confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM). Significantly, a modified sample pretreatment technique was employed to demonstrate clearly the emulsion phase interface on the SEM.Innovative FCAGP-stabilized Pickering emulsions achieved 65.39 % in DMY bioavailability, facilitating its slow-release and enhanced bioaccessibility during in vitro digestion. Pickering emulsions loaded DMY demonstrated improved stability and sustained release under simulated digestive conditions, bolstering its therapeutic efficacy. Our findings solidify the theoretical basis for green synthesis of propeptide-polysaccharide nanoparticles and introduce a novel strategy for targeted propeptide Pickering emulsions in drug delivery, highlighting the transformative potential of FCAGP nanoparticles in enhancing lipophilic drug bioavailability and pharmacological impact. [Display omitted] • Surface Hydrophobicity: Protein surface hydrophobicity enhanced by Ampelopsis grossedentata polysaccharide modification. • Pickering Emulsion Stability: AGP significantly improves the storage stability of Pickering emulsions. • Interfacial Dynamics Insight: Advanced styrene/V65 technology reveals new details of emulsion phase interfaces. • Enhanced Bioavailability: Pickering emulsions increase dihydromyricetin bioavailability as a delivery system. [ABSTRACT FROM AUTHOR]