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Start Over Topic early detection of cancer Journal communications biology
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1. An origami paper-based nanoformulated immunosensor detects picograms of VEGF-C per milliliter of blood.

Author

Sun S, Wang Y, Ming T, Luo J, Xing Y, Liu J, Xiong Y, Ma Y, Yan S, Yang Y, and Cai X

Subjects
Biomarkers, Tumor analysis, Biomarkers, Tumor blood, Blood Chemical Analysis instrumentation, Blood Chemical Analysis methods, Electrochemical Techniques instrumentation, Electrochemical Techniques methods, Electrodes, Gold chemistry, Humans, Immunoassay instrumentation, Immunoassay methods, Lab-On-A-Chip Devices, Limit of Detection, Metal Nanoparticles chemistry, Microtechnology, Nanocomposites chemistry, Nanotubes, Carbon chemistry, Neoplasms blood, Neoplasms diagnosis, Paper, Vascular Endothelial Growth Factor C analysis, Biosensing Techniques instrumentation, Biosensing Techniques methods, Early Detection of Cancer instrumentation, Early Detection of Cancer methods, Vascular Endothelial Growth Factor C blood
Abstract

Detecting vascular endothelial growth factor C (VEGF-C), a kind of tumor biomarker, is of significant clinical importance in evaluating the prognosis of patients with cancer. However, laboratory analyses are usually not suitable for point-of-care testing because they are expensive and time consuming. In response to these challenges, we fabricated an origami paper-based microfluidic electrochemical device. To improve the specificity of VEGF-C detection, nanocomposites, synthesized by new methylene blue (NMB), amino-functional single-walled carbon nanotubes (NH 2 -SWCNTs), and gold nanoparticles (AuNPs), were used to modify the surface of working electrodes. Results of electrochemical detection showed that the immunosensor had excellent linearity, ranging from 0.01 to 100 ng mL -1 (R 2  = 0.988), and the limit of detection was 10 pg mL -1 . To confirm the high specificity of the device under real-world conditions, we evaluated the device using clinical serum samples from our hospital. The results demonstrated that the device had an excellent performance and could provide a platform for real-time detection of cancers.

Published
2021
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2. An origami paper-based nanoformulated immunosensor detects picograms of VEGF-C per milliliter of blood

Author

Yu Xing, Tao Ming, Jinping Luo, Shi Yan, Yuanyuan Ma, Juntao Liu, Yue Yang, Ying Xiong, Yang Wang, Shuai Sun, and Xinxia Cai

Subjects
Paper, QH301-705.5, VEGF receptors, Microfluidics, Vascular Endothelial Growth Factor C, Medicine (miscellaneous), Nanoparticle, Metal Nanoparticles, 02 engineering and technology, Carbon nanotube, Biosensing Techniques, 010402 general chemistry, 01 natural sciences, General Biochemistry, Genetics and Molecular Biology, Article, law.invention, Nanocomposites, Cancer screening, Tumour biomarkers, chemistry.chemical_compound, Blood serum, law, Limit of Detection, Lab-On-A-Chip Devices, Neoplasms, Biomarkers, Tumor, Humans, Biology (General), Electrodes, Early Detection of Cancer, Detection limit, Immunoassay, biology, Chemistry, Nanotubes, Carbon, New methylene blue, Electrochemical Techniques, 021001 nanoscience & nanotechnology, 0104 chemical sciences, Colloidal gold, biology.protein, Microtechnology, Nanoparticles, Gold, 0210 nano-technology, General Agricultural and Biological Sciences, Blood Chemical Analysis, Biomedical engineering
Abstract

Detecting vascular endothelial growth factor C (VEGF-C), a kind of tumor biomarker, is of significant clinical importance in evaluating the prognosis of patients with cancer. However, laboratory analyses are usually not suitable for point-of-care testing because they are expensive and time consuming. In response to these challenges, we fabricated an origami paper-based microfluidic electrochemical device. To improve the specificity of VEGF-C detection, nanocomposites, synthesized by new methylene blue (NMB), amino-functional single-walled carbon nanotubes (NH2-SWCNTs), and gold nanoparticles (AuNPs), were used to modify the surface of working electrodes. Results of electrochemical detection showed that the immunosensor had excellent linearity, ranging from 0.01 to 100 ng mL−1 (R2 = 0.988), and the limit of detection was 10 pg mL−1. To confirm the high specificity of the device under real-world conditions, we evaluated the device using clinical serum samples from our hospital. The results demonstrated that the device had an excellent performance and could provide a platform for real-time detection of cancers., Sun, Wang et al. report an origami paper-based immunosensor for the electrochemical detection of the VEGF-C biomarker in blood serum. The immunosensor is made by modifying the surface of working electrodes with new methylene blue, amino-functional single-walled carbon nanotubes, and gold nanoparticles and demonstrates excellent performance with a limit of detection in the range of picograms per milliliter.

Published
2021