1. Identification and characterization of two phospholipid hydroperoxide glutathione peroxidase genes from Apis cerana cerana
- Author
-
Wang, Mian, Kang, Mingjiang, Guo, Xingqi, and Xu, Baohua
- Subjects
- *
PHOSPHOLIPIDS , *PEROXIDASE , *APIS cerana , *BIOLOGICAL membranes , *EXONS (Genetics) , *INSECT larvae , *GENE expression , *REVERSE transcriptase polymerase chain reaction - Abstract
Abstract: Phospholipid hydroperoxide glutathione peroxidase (PHGPX) plays a crucial role in maintaining the integrity of membrane by reducing hydroperoxides of phospholipids. Here, we report the identification and characterization of two genes, designated AccGtpx-1 and AccGtpx-2, encoding PHGPX proteins from the Chinese honeybees, Apis cerana cerana. Alignment analysis showed that AccGtpx-1 and AccGtpx-2 shared high similarity with other known PHGPXs, which show similar structure to thioredoxin. These single copy genes showed complex exon–intron structures. The mRNA of AccGtpx-1 was detected in larvae, pupae and adults and that AccGtpx-2 was only found in adult worker bees. Furthermore, the expression of AccGtpx-1 could be induced by H2O2, ultraviolet (UV) light, heat shock (37°C), HgCl2, imidacloprid, cyhalothrin, pyriproxyfen and methomyl. In contrast, AccGtpx-2 expression could only be induced by UV. These results indicated for the first time that the AccGtpx-1 and AccGtpx-2 genes encoding A. cerana cerana PHGPXs are regulated differently in response to environmental stressors. [Copyright &y& Elsevier]
- Published
- 2010
- Full Text
- View/download PDF