1. Purification and characterization of vitellogenin from the American cockroach, Periplaneta americana
- Author
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Hak R. Kim, Chihiro Katagiri, Haruo Chino, and Eriko Nagao
- Subjects
Cockroach ,Chromatography ,Molecular mass ,Physiology ,General Medicine ,Biology ,biology.organism_classification ,Biochemistry ,Vitellogenin ,biology.animal ,Hemolymph ,biology.protein ,Density gradient ultracentrifugation ,Vitellogenesis ,American cockroach ,Molecular Biology ,Periplaneta - Abstract
1. 1. Vitellogenin (VG) was isolated and purified from the hemolymph of female American cockroaches. 2. 2. The purification method used in this study comprises two steps: the first step is based on the method originally developed for purifying lipophorin from hemolymph, and the second step is the separation of VG from lipophorin by a KBr density gradient ultracentrifugation. 3. 3. The purified VG was characterized according to molecular weight, substructure, shape and size, and lipid composition. 4. 4. The VG molecule is almost globular in shape with the diameter of about 15.5 nm and is indistinguishable from lipophorin in shape and size. 5. 5. The native molecular weight determined by light scattering method was 560 kDa. 6. 6. The VG consists of four subunits with molecular weights of approximately 102, 81, 49 and 40 kDa, respectively. 7. 7. VG is a lipoprotein and comprises 92% protein and 8% lipid. 8. 8. Major lipid components were found to be diacylglycerol (25%) and phospholipids (71%).
- Published
- 1992
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