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Introduction The objective of the present study was to identify gene expression in peripheral blood by a real-time polymerase chain reaction (PCR) technique in patients who have lung carcinoma. Material and methods Peripheral blood samples of patients with non-small cell and small cell lung cancer were collected. Target genes included survivin, CK7, ASH1, HMGB3, L587S, and CLCA2. β-Actin was the reference gene. If the mean C T (threshold cycle) value for a target gene is ≥40, the gene expression is considered undetectable. Results Fifty patients with lung carcinoma were included and 30 healthy controls. Out of the six genes, survivin showed 26.8 times fold change as compared to controls; ASH1 and L587S were 0.54 and 0.06, respectively; and HMGB3, CLCA2, and CK7 had non-significant fold change in comparison to controls. The overall detection rate of the six target genes examined in lung cancer was 84%, with 42 out of 50 patients testing positive. Higher stages and ASH1 (p = 0.031), CK7 (p = <0.001), and HMGB3, p = 0.011 were associated significantly. CLCA2 had higher expression in patients without adrenal metastases (p = 0.044). Conclusions Lifestyle and geographical variation might be a probable cause of variable gene expression as compared to other studies. However, further research is needed to determine the clinical implication of these markers, especially in larger groups of early-stage patients., Competing Interests: Human subjects: Consent was obtained or waived by all participants in this study. All India Institute of Medical Sciences, Rishikesh issued approval 83/IEC/EM/2016. The study was approved by the Institutional Ethics Committee (83/IEC/EM/2016). Animal subjects: All authors have confirmed that this study did not involve animal subjects or tissue. Conflicts of interest: In compliance with the ICMJE uniform disclosure form, all authors declare the following: Payment/services info: Grant received: This study has been funded by the Department of Science and Technology New Delhi under the WOS-A Project (SR/WOS-A/LS-605/2016(G). Financial relationships: All authors have declared that they have no financial relationships at present or within the previous three years with any organizations that might have an interest in the submitted work. Other relationships: All authors have declared that there are no other relationships or activities that could appear to have influenced the submitted work., (Copyright © 2024, Gupta et al.)

Background: Decreased diet intake and malnourishment have profound implications on cancer patients' quality of life and survival. Malnutrition increases the risk of postoperative complications, increases hospital length stays, reduces patient's tolerance to radiation and chemotherapy treatment, and results in poor response to treatment. In the present study, we intended to assess the nutritional status of cancer patients and find the correlation of body mass index with anthropometric and blood parameters., Material & Methods: The study was prospective and cross-sectional, and 104 patients with newly diagnosed solid tumors were included. Patient demographics, symptoms, and anthropometric and blood parameters were collected. The correlation was estimated with Pearson's correlation coefficient. A p-value of less than 0.05 was considered significant., Results: The association between stages of the disease, dental status, type of diet, and BMI was p=0.701, 0.216, and 0.422, respectively, and was not statistically significant. The anthropometric parameters mid upper arm circumference (MUAC cm), mid arm circumference (MAC cm), and triceps skinfold thickness (TSF mm) correlated with body mass index (BMI kg/m 2 ) and had statistically significant p values of 0.0001, 0.0001, and 0.033, respectively. The correlation was assessed between hemoglobin, red cell distribution width, neutrophil-to-lymphocyte ratio, and serum albumin levels with BMI, but except for albumin (p=0.05), no other blood parameter correlated., Conclusion: Nutritional assessment is vital in recognizing patients at risk of treatment-associated complications and poor responders to treatment. In this study, BMI correlated with anthropometric parameters MUAC, MAMC, and TSF. Baseline dietary assessments of patients will help focus on the nutritional build-up of patients before starting treatment., Competing Interests: The authors have declared that no competing interests exist., (Copyright © 2024, Vidhya et al.)

Background: Poisoning is a major health issue worldwide. Nurses are frequently the first healthcare professionals to come in contact with patients with acute poisoning. So, this study was conducted to assess the knowledge and awareness regarding the management of acute poisoning among nurses. In case of insufficient knowledge, they could be trained enough to manage any case of acute poisoning for life-saving purposes., Methodology: This was a cross-sectional analytical study conducted among 212 nurses at Rajendra Institute of Medical Sciences, Ranchi, Jharkhand from July 2021 to December 2021. Data were collected using the structured questionnaire based on a previous study and regression analysis was used for determining the association between their overall knowledge score and independent variables like age, education, experience, and training status., Results: More than one-third (43.8%) of the study participants had an unsatisfactory level of knowledge, which might be because of a lack of proper training and experience, but still, 42.4% and 13.6% of the study participants had satisfactory and good knowledge, respectively. Moreover, more than one-third (45.2%) of the study participants had inefficient skills, while 48.1% and 6.6% of nurses had satisfactory and good skills, respectively, in the management of acute poisoning. The three independent variables (training, experience, and education) showed a 53.1% association with total scores in bivariate regression analysis while no significant association was seen between age and score in multivariate analysis., Conclusions: About half of the nurses showed unsatisfactory knowledge, so they need improvement and they should be trained regarding this to reduce mortality among acute poisoning cases., Competing Interests: The authors have declared that no competing interests exist., (Copyright © 2023, Kumari et al.)