1. A Casein Hydrolysate Does Not Enhance Ileal Endogenous Protein Flows Compared With the Parent Intact Casein When Fed to Growing Pigs
- Author
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Amélie Deglaire, Daniel Tomé, Paul J. Moughan, Science et Technologie du Lait et de l'Oeuf (STLO), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Massey University, Physiologie de la Nutrition et du Comportement Alimentaire (PNCA), AgroParisTech-Institut National de la Recherche Agronomique (INRA), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), and Institut National de la Recherche Agronomique (INRA)-AgroParisTech
- Subjects
pig ,modèle animal ,030309 nutrition & dietetics ,Medicine (miscellaneous) ,Ileum ,Endogeny ,digestion ,ileal ,allégations nutritionnelles et de santé ,casein ,03 medical and health sciences ,Suidae ,intestin ,Casein ,[SDV.IDA]Life Sciences [q-bio]/Food engineering ,endogenous ,medicine ,animal modèle ,Nutrition and Intestinal Physiology ,Centrifugation ,Dry matter ,Food science ,Original Research ,2. Zero hunger ,chemistry.chemical_classification ,0303 health sciences ,amino acids ,Nutrition and Dietetics ,caséine ,biology ,Chemistry ,0402 animal and dairy science ,04 agricultural and veterinary sciences ,biology.organism_classification ,040201 dairy & animal science ,animal models ,Amino acid ,acide aminé ,activité intestinale ,medicine.anatomical_structure ,protéine ,Digestion ,protein ,[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition ,amino acid ,Food Science - Abstract
Background The form of dietary nitrogen (free peptides or intact proteins) may influence the amount of endogenous amino acids found at the terminal ileum of the pig, and it has been speculated that hydrolyzed dietary protein may lead to increased endogenous amino acids. Objective To compare the effect of dietary free peptides on ileal endogenous nitrogen and amino acid flows [ileal endogenous nitrogen flow (ENFL), ileal endogenous amino acid flow (EAAFL)] with that of peptides released naturally from dietary protein during digestion, from the same intact parent protein source. Methods Six pigs (mean body weight: 34 kg) were equipped with a postvalve T-caecum cannula. Semisynthetic test diets contained the same 15N-labeled intact casein (C) or hydrolyzed casein (HC). Pigs received the test diets every sixth day and the corresponding unlabeled diets in the intervening 5-d periods. Digesta were pooled from 4 to 10 h postprandially. EAAFL and ENFL, calculated with reference to the dietary marker titanium dioxide, were determined by isotope dilution for C and HC. Results Ileal EAAFL and ENFL (mean flows n = 5 of 1828 and 1912 μg/g of dry matter intake for diets HC and C, respectively) did not differ (P > 0.05) between pigs fed HC and C. Centrifugation and ultrafiltration of the HC digesta allowed an estimation of label recycling into gut endogenous proteins. Some 20% of ileal endogenous protein (diet HC, ultrafiltered digesta) was 15N-labeled due to tracer recycling. Conclusions The administration of a casein hydrolysate had no effect on ileal endogenous protein flows compared with C. There was no evidence of enhanced ileal endogenous protein losses with the HC diet.
- Published
- 2018