1. Antioxidant Activity and Role of Culture Condition in the Optimization of Red Pigment Production by Talaromyces purpureogenus KKP Through Response Surface Methodology
- Author
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Rajesh P. Shastry, Prashant Kumar Modi, Kishor Kumar Keekan, and Sweta Hallur
- Subjects
0303 health sciences ,Talaromyces purpureogenus ,Limonene ,Antioxidant ,Central composite design ,030306 microbiology ,medicine.medical_treatment ,General Medicine ,Biology ,Industrial microbiology ,Applied Microbiology and Biotechnology ,Microbiology ,03 medical and health sciences ,Pigment ,chemistry.chemical_compound ,chemistry ,visual_art ,visual_art.visual_art_medium ,medicine ,Food science ,Response surface methodology ,Delphinidin ,030304 developmental biology - Abstract
The red pigment production by Talaromyces purpureogenus KKP, a soil isolate, was optimized by response surface methodology (RSM) in the present study. The cultural parameters, such as pH, temperature, dextrose, and peptone concentrations, were optimized for red pigment production using the central composite design (CCD) experimental design. A second-order quadratic model was used to calculate the relationships between the values at different levels of response. The optimum values of the selected variables under coded factors are 6.0, 27 °C, 2.25%, and 1.10% for pH, temperature, dextrose, and peptone, respectively. The selected variables were most effective in the enhancement of red pigment production at optimized culture conditions. In addition to optimization, the antioxidant activity of the pigment isolated in the present study was found to be promising with IC50 value (40 µg/ml). The HRMS data revealed the identification of delphinidin, limonene, 6-hydroxymethyl-7,8-dihydropterin, d-mannose 6-phosphate, and CDP-DG (18:0/18:0). The results of the present investigation will be added to the existing literature of red pigment production and its optimization by T. purpureogenus
- Published
- 2020