1. Organic phase separation opens up new opportunities to interrogate the RNA-binding proteome
- Author
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Charlotte Dawson, Jeroen Krijgsveld, Mohamed Elzek, Anne E. Willis, Kathryn S. Lilley, Rayner M. L. Queiroz, Eneko Villanueva, Tom Smith, Benedikt M. Beckmann, and Erika C. Urdaneta
- Subjects
Proteomics ,0301 basic medicine ,RNA metabolism ,Proteome ,Chemistry ,RNA-Binding Proteins ,RNA ,RNA-binding protein ,Limiting ,Computational biology ,010402 general chemistry ,01 natural sciences ,Biochemistry ,0104 chemical sciences ,Analytical Chemistry ,Transcriptome ,03 medical and health sciences ,030104 developmental biology ,RNA, Messenger ,Function (biology) ,Ribonucleoprotein - Abstract
Protein–RNA interactions regulate all aspects of RNA metabolism and are crucial to the function of catalytic ribonucleoproteins. Until recently, the available technologies to capture RNA-bound proteins have been biased toward poly(A) RNA-binding proteins (RBPs) or involve molecular labeling, limiting their application. With the advent of organic–aqueous phase separation–based methods, we now have technologies that efficiently enrich the complete suite of RBPs and enable quantification of RBP dynamics. These flexible approaches to study RBPs and their bound RNA open up new research avenues for systems-level interrogation of protein–RNA interactions.
- Published
- 2020
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