1. Quantitative bacterial transcriptomics with RNA-seq
- Author
-
James P. Creecy and Tyrrell Conway
- Subjects
Genetics, Microbial ,Microbiology (medical) ,Regulation of gene expression ,Genetics ,Bacteria ,Operon ,Gene Expression Profiling ,High-Throughput Nucleotide Sequencing ,RNA ,RNA-Seq ,Promoter ,Gene Expression Regulation, Bacterial ,Computational biology ,MOLECULAR BIOLOGY METHODS ,Biology ,Microbiology ,Article ,Transcriptome ,Infectious Diseases ,RNA Sequence ,Molecular Biology - Abstract
RNA sequencing has emerged as the premier approach to study bacterial transcriptomes. While the earliest published studies analyzed the data qualitatively, the data are readily digitized and lend themselves to quantitative analysis. High-resolution RNA sequence (RNA-seq) data allows transcriptional features (promoters, terminators, operons, among others) to be pinpointed on any bacterial transcriptome. Once the transcriptome is mapped, the activity of transcriptional features can be quantified. Here we highlight how quantitative transcriptome analysis can reveal biological insights and briefly discuss some of the challenges to be faced by the field of bacterial transcriptomics in the near future.
- Published
- 2015
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