1. Bacterial replisomes.
- Author
-
Xu ZQ and Dixon NE
- Subjects
- Bacillus subtilis enzymology, Bacillus subtilis genetics, Bacteriophage T7 enzymology, Bacteriophage T7 genetics, DNA Helicases chemistry, DNA Helicases metabolism, DNA Ligases chemistry, DNA Ligases metabolism, DNA Polymerase I chemistry, DNA Polymerase I metabolism, DNA Polymerase III chemistry, DNA Polymerase III metabolism, DNA-Directed DNA Polymerase chemistry, DNA-Directed DNA Polymerase metabolism, Helicobacter pylori enzymology, Helicobacter pylori genetics, DNA Replication, Escherichia coli enzymology, Escherichia coli genetics, Multienzyme Complexes chemistry, Multienzyme Complexes metabolism
- Abstract
Bacterial replisomes are dynamic multiprotein DNA replication machines that are inherently difficult for structural studies. However, breakthroughs continue to come. The structures of Escherichia coli DNA polymerase III (core)-clamp-DNA subcomplexes solved by single-particle cryo-electron microscopy in both polymerization and proofreading modes and the discovery of the stochastic nature of the bacterial replisomes represent notable progress. The structures reveal an intricate interaction network in the polymerase-clamp subassembly, providing insights on how replisomes may work. Meantime, ensemble and single-molecule functional assays and fluorescence microscopy show that the bacterial replisomes can work in a decoupled and uncoordinated way, with polymerases quickly exchanging and both leading-strand and lagging-strand polymerases and the helicase working independently, contradictory to the elegant textbook view of a highly coordinated machine., (Copyright © 2018 The Authors. Published by Elsevier Ltd.. All rights reserved.)
- Published
- 2018
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