1. Toxicity of some charged lipids used in liposome preparations.
- Author
-
Campbell PI
- Subjects
- Animals, Cell Division drug effects, Cholesterol toxicity, DNA metabolism, Leukemia L1210 metabolism, Mice, Phosphatidic Acids toxicity, Phosphatidylcholines toxicity, Phosphatidylserines toxicity, Thymidine metabolism, Lipids toxicity, Liposomes toxicity
- Abstract
The inhibition of 3H-thymidine incorporation into DNA of L1210 cells in culture by liposomes was used as an index of liposome toxicity. Inhibition of 3H-thymidine incorporation appeared to be dose dependent for some lipid compositions tested. The commonly used neutral lipids, phosphatidylcholine and cholesterol did not appear to inhibit 3H-thymidine incorporation. Phosphatidic acid, an adjunct for preparing anionic liposomes, appeared to be non-toxic compared to phosphatidylserine and dicetylphosphate (alternative adjuncts for preparing anionic liposomes). Stearylamine, a synthetic lipid which continued to dominate the preparation of cationic liposome was the most toxic of the lipids tested.
- Published
- 1983