1. Physical mapping of the CXC chemokine locus on human chromosome 4.
- Author
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O'Donovan, N., Galvin, M., and Morgan, J. G.
- Subjects
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CHEMOKINES , *HUMAN gene mapping , *POLYMERASE chain reaction , *LEUKEMIA inhibitory factor , *HUMAN chromosomes , *INFLAMMATORY mediators , *CYSTEINE proteinases - Abstract
A physical map of the CXC chemokine locus on chromosome 4 has been constructed by PCR analysis and PFGE mapping of YAC clones. The genes for IL8, GRO1, PPBP, PF4, SCYB5 (ENA-78) and SCYB6 (GCP-2) have been co-localized on a 335-kb genomic fragment. The GRO2 and GRO3 genes did not map within this region and based on analysis of a YAC contig overlapping IL8 we speculate that GRO2 and GRO3 map downstream of this region. We have also assigned the novel CXC chemokine gene, SCYB9B (alias H174/βR1) to chromosome 4q21, upstream and within 12 kb of INP10. Like INP10 and MIG, INP10 and SCYB9B are arranged in a head to tail manner. The chromosomal arrangement of these genes appears to reflect the evolution of this multigene family and supports the theory that it arose by gene duplication. [ABSTRACT FROM AUTHOR]
- Published
- 1999
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