1. Automatic telomere length measurements in interphase nuclei by IQ-FISH.
- Author
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Narath R, Lörch T, Greulich-Bode KM, Boukamp P, and Ambros PF
- Subjects
- Adult, Blotting, Southern, Cell Line, Tumor, Data Interpretation, Statistical, Fluorescent Dyes, Humans, Leukocytes, Mononuclear cytology, Leukocytes, Mononuclear ultrastructure, Metaphase, Neuroblastoma ultrastructure, Osteosarcoma ultrastructure, Cell Nucleus ultrastructure, In Situ Hybridization, Fluorescence methods, Interphase, Microscopy, Fluorescence methods, Telomere ultrastructure
- Abstract
To benefit from the fluorescence-based automatic microscope (FLAME), we have adapted a PNA FISH technique to automatically determine telomere length in interphase nuclei. The method relies on the simultaneous acquisition of pan-telomeric signals and reference probe signals. We compared the quantitative figures to those for existing methods, i.e. Southern blot analysis and quantitative FISH (Q-FISH). Quantitative-FISH on interphase nuclei (IQ-FISH) allows the exact quantification of telomere length in interphase nuclei. Thus, this enables us to obtain not only exact information on the telomere length, but also morphological and topological details. The automatic measurement of large cell numbers allows the measurement of statistically relevant cell populations.
- Published
- 2005
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