1. An Evaluation of T-Cell Functionality After Flow Cytometry Sorting Revealed p38 MAPK Activation
- Author
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Lynette Henkel, Immanuel Andrä, Julia Ritter, Sabine Przibilla, Corinne Angerpointner, Hanna Ulrich, Herbert Stadler, Matthias Schiemann, Dominik Soll, Dirk H. Busch, Manuel Effenberger, and Susi Durr
- Subjects
0301 basic medicine ,MAPK/ERK pathway ,Cell signaling ,Histology ,T cell ,p38 mitogen-activated protein kinases ,T-Lymphocytes ,Cell ,Cell Separation ,p38 Mitogen-Activated Protein Kinases ,Pathology and Forensic Medicine ,Flow cytometry ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Humans ,medicine.diagnostic_test ,Chemistry ,Cell Biology ,Cell sorting ,Flow Cytometry ,ddc ,Cell biology ,030104 developmental biology ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Cytometry ,Signal Transduction - Abstract
Cell alterations during isolation and preparation for flow cytometry cell sorting by antibodies, temperature, homogenization, buffer composition and mitogens are well known. In contrast, little is known about cell alteration caused by the instrument or the sorting process itself. We systematically evaluated cellular responses to different sorter-induced physical forces. In summary, flow cytometry cell-sorting induced forces can affect cellular signaling cascades, especially the MAPK p38. Functional assays, related to the p38 MAPK pathway, of human primary T cells after flow cytometry sorting did lead to minor physiological modulation but no functional impairments. © 2020 The Authors. Cytometry Part A published by Wiley Periodicals, Inc. on behalf of International Society for Advancement of Cytometry.
- Published
- 2019