Search

Your search keyword '"Kengo Horiuchi"' showing total 1 results
Start Over Author "Kengo Horiuchi" Journal cytotechnology
1 results on '"Kengo Horiuchi"'

1. Reduced immunogenicity and endowed antimicrobial activity in β-lactoglobulin by preparing edible bioconjugate

Author

Tadashi Yoshida, Risa Ikeda, Shinya Kawai, Kengo Horiuchi, Yuho Matsuo, and Makoto Hattori

Subjects
Clinical Biochemistry, Biomedical Engineering, Bioengineering, Cell Biology, Biotechnology
Abstract

β-lactoglobulin (BLG) and ε-polylysine (PL) were bound by using microbial transglutaminase. Dextran (Dex) was further conjugated to the BLG-PL conjugate by the Maillard reaction. Confirmation of conjugation was carried out by SDS-PAGE. From the results of isoelectric focusing, it was revealed that the isoelectric point of the BLG-PL conjugate was shifted to the basic side as compared with native BLG. Immunogenicity of BLG in BALB/c mice was lowered by conjugation with PL and further lowered by conjugation with Dex. By conjugation with PL and PL-Dex, antibacterial activity against S. aureus was endowed to BLG. Because the conjugation method in this study is a safe method, it is valuable in that it can be applicable to food processing.

Published
2022

Catalog

Books, media, physical & digital resources
See catalog results