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1. ILDS Newsletter No. 32

Author

Bruna A. Pereira, Xin Li, Stefan Kippenberger, Chuen Seng Tan, Simone Cazzaniga, Enzo Berardesca, Riccardo Sirna, Jun Young Lee, Dario Fai, Marco Ardigò, José Antonio Sanches, Yoonhee Lee, Wei Li, Marianne Chopinaud, Chantal Andre, Shu Jun Wang, Charlotte Gourio, Wen Jie Zhang, Won-Soo Lee, Laurence Verneuil, Natalie Garcia Bartels, Emilie Sbidian, Yang Lu, Enrico Pezzarossa, Yun Pei Koh, Saskia Ingen-Housz-Oro, Maria Letizia Musumeci, Ulrich Mrowietz, Maria Donata Digiuseppe, Manuel Butting, Claudia Grande, Anne Dompmartin, Werner Druck Medien Ag, Pierre Wolkenstein, N. Bellon, Sascha Gerdes, Philippe Rochaix, Varvara Kanti, Eva M. Valesky, R.M. Penín, Nadja Zöller, Diana Pérez, Yoke Chin Giam, Vito Ingordo, Amelie Clementine Seghers, Nicolas Meyer, Vito Di Lernia, Daniel Labbé, Jürgen Bereiter-Hahn, Christine Coutanceau, Yen Loo Lim, Carlo Donadio, Catia De Felice, Ji Hoon Chun, Michael B Halstead, Ji Hye Baek, Melisa Barrantes-González, Joyce Siong See Lee, Anne-Dominique Pham, Jean-François Stalder, Wen-Juan Wu, Hervé Bénateau, Ya-Ping Zhang, Andrea Stroux, Hee Chul Eun, Vincenzo Claudio Battarra, Catherine Gentil, Matelda Medri, Carle Paul, August Bernd, Olivier Chosidow, Michele Pellegrino, Satz Mengensatzproduktion, Beatrice Raone, Mercè Espona-Quer, Kirsten Steinz, Seung Hwan Paik, Oh Sang Kwon, Joaquim Marcoval, Li Hu, Mark B Y Tang, Hye Chan Jeon, Jian-Kang Yang, Li He, Josep Ramon Ferreres, Raphael Lopez, Fabricio C. Furlan, Marina Agozzino, Ulrike Blume-Peytavi, Claudia Cavallotti, Ivana Romano, Nicolas Ortonne, Francesca Peccerillo, Jin Yong Kim, Ignacio Garrido, Roland Kaufmann, Luigi Naldi, Ana Giménez-Arnau, Madeline S.L. Ho, Christine Chevreau, Annalisa Patrizi, Coline Munsch, Isabella Sperduti, Esther Salas, Silja Domm, Christoph Bührer, Matthias Hofmann, Chiara Franceschini, Laurence Lamant, John D. Rogers, Mirian Nacagami Sotto, Joan Maria Viñals, Ye-Jin Jung, and Valérie Lauwers-Cances

Subjects
medicine.medical_specialty, business.industry, Medicine, Dermatology, business
Published
2014

2. Clinical Application of a Tissue-Cultured Skin Autograft: An Alternative for the Treatment of Non-Healing or Slowly Healing Wounds?

Author

Matthias Hofmann, Stefan Kippenberger, Eva Maria Valesky, August Bernd, Roland Kaufmann, Nadja Zöller, Jürgen Bereiter-Hahn, and Manuel Butting

Subjects
Keratinocytes, medicine.medical_specialty, Treatment outcome, Dermatology, Risk Assessment, Severity of Illness Index, Artificial skin, Tissue Culture Techniques, Tissue scaffolds, Skin Ulcer, medicine, Humans, Autografts, Healing wounds, Aged, Wound Healing, Cultured skin, Tissue Engineering, Tissue Scaffolds, Treatment regimen, business.industry, Biopsy, Needle, Graft Survival, Follow up studies, Skin Transplantation, Fibroblasts, Immunohistochemistry, Surgery, Treatment Outcome, Face, Female, Graft survival, business, Follow-Up Studies
Abstract

Background: The treatment regime of non-healing or slowly healing wounds is constantly improving. One aspect is surgical defect coverage whereby mesh grafts and keratinocyte suspension are applied. Objective: Tissue-cultured skin autografts may be an alternative for the treatment of full-thickness wounds and wounds that cover large areas of the body surface. Methods: Autologous epidermal and dermal cells were isolated, expanded in vitro and seeded on collagen-elastin scaffolds. The developed autograft was immunohistochemically characterized and subsequently transplanted onto a facial chronic ulceration of a 71-year-old patient with vulnerable atrophic skin. Results: Characterization of the skin equivalent revealed comparability to healthy human skin due to the epidermal strata, differentiation and proliferation markers. Within 138 days, the skin structure at the transplantation site closely correlated with the adjacent undisturbed skin. Conclusion: The present study demonstrates the comparability of the developed organotypic skin equivalent to healthy human skin and the versatility for clinical applications.

Published
2014

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