1. Removal and biotransformation of U(VI) and Cr(VI) by aerobically grown mixed microbial granules.
- Author
-
Nancharaiah, Y. Venkata, Venugopalan, V.P., and Francis, A.J.
- Subjects
BIOTRANSFORMATION (Metabolism) ,SEWAGE sludge ,BIOREMEDIATION ,MOLECULAR orbitals ,X-ray absorption near edge structure ,MATHEMATICAL models ,BATCH reactors - Abstract
We assessed the potential of aerobic granular sludge consisting of mixed species of bacteria to remove and immobilize uranium (VI) and chromium (VI). Microbial granules were cultivated in a laboratory sequencing batch reactor (SBR) by feeding with acetate-containing synthetic media. Microbial granules formed in the SBR exhibited excellent settling characteristics and predominantly consisted of rod/cocci shaped bacteria. The microbial granules immobilized 218 ± 2 mg of U(VI) g
-1 dry granular biomass. X-ray photoelectron spectroscopy (XPS) showed the association of U(VI) with the microbial granules and transformation of U(VI) to U(IV). Microbial granules reduced Cr(VI) and immobilized to Cr(III) at 0.17 mmoles/d/g under anaerobic conditions. X-ray absorption near edge spectroscopy (XANES) of chromium associated with microbial granules revealed complete conversion of Cr(VI) to Cr(III). Extended X-ray absorption fi ne structure (EXAFS) analysis of the Cr-laden microbial granules showed similarity to Cr(III)-phosphate. This study demonstrates the biotransformation and immobilization of U(VI) and Cr(VI) by mixed species microbial granules. [ABSTRACT FROM AUTHOR]- Published
- 2012
- Full Text
- View/download PDF