1. TRPP2-dependent Ca2+ signaling in dorso-lateral mesoderm is required for kidney field establishment in Xenopus
- Author
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Muriel Umbhauer, Jean-François Riou, Isabelle Buisson, Marc Moreau, Isabelle Néant, Mélinée Futel, Ronan Le Bouffant, Catherine Leclerc, Signalisation et morphogenèse = Signalling and morphogenesis (LBD-E12), Laboratoire de Biologie du Développement (LBD), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), CNRS, Universite Pierre et Marie Curie (UPMC), Emergence-UPMC research program, `Complexite du Vivant' doctoral school, Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), and Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
Embryo, Nonmammalian ,TRPP Cation Channels ,Field (physics) ,Xenopus ,Retinoic acid ,Kidney field specification ,Xenopus Proteins ,Biology ,Kidney ,Pronephros ,chemistry.chemical_compound ,Transient receptor potential channel ,PAX8 Transcription Factor ,Xenopus laevis ,03 medical and health sciences ,0302 clinical medicine ,Downregulation and upregulation ,Extracellular ,medicine ,Animals ,Paired Box Transcription Factors ,Calcium Signaling ,Ca2+ signaling ,[SDV.BDD]Life Sciences [q-bio]/Development Biology ,Molecular Biology ,030304 developmental biology ,0303 health sciences ,urogenital system ,Lateral plate mesoderm ,Cell Biology ,Anatomy ,biology.organism_classification ,Molecular biology ,Pax8 ,Cell biology ,medicine.anatomical_structure ,chemistry ,030217 neurology & neurosurgery ,Ca2 signaling ,TRPP2 ,Developmental Biology - Abstract
In Xenopus laevis embryos, kidney field specification is dependent on retinoic acid (RA) and coincides with a dramatic increase of Ca 2+ transients, but the role of Ca 2+ signaling in the kidney field is unknown. Here, we identify TRPP2, a member of the transient receptor potential (TRP) superfamily of channel proteins encoded by the pkd2 gene, as a central component of Ca 2+ signaling in the kidney field. TRPP2 is strongly expressed at the plasma membrane where it might regulate extracellular Ca 2+ entry. Knockdown of pkd2 in the kidney field results in the downregulation of pax8 , but not of other kidney field genes ( lhx1 , osr1 and osr2 ). We further show that inhibition of Ca 2+ signaling with an inducible Ca 2+ chelator also causes downregulation of pax8 , and that pkd2 knockdown results in a severe inhibition of Ca 2+ transients in kidney field explants. Finally, we show that disruption of RA results both in an inhibition of intracellular Ca 2+ signaling and of TRPP2 incorporation into the plasma membrane of kidney field cells. We propose that TRPP2-dependent Ca 2+ signaling is a key component of pax8 regulation in the kidney field downstream of RA-mediated non-transcriptional control of TRPP2.
- Published
- 2015
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