1. Development of the mammary gland requires DGAT1 expression in stromal and epithelial tissues.
- Author
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Cases S, Zhou P, Shillingford JM, Wiseman BS, Fish JD, Angle CS, Hennighausen L, Werb Z, and Farese RV Jr
- Subjects
- Animals, Apoptosis, Cell Differentiation, Diacylglycerol O-Acyltransferase, Image Processing, Computer-Assisted, Immunoblotting, Immunohistochemistry, Lactation, Lipid Metabolism, Mice, Mice, Inbred C57BL, Mice, Transgenic, Precipitin Tests, RNA metabolism, Time Factors, Tissue Transplantation, Acyltransferases biosynthesis, Acyltransferases physiology, Epithelial Cells metabolism, Mammary Glands, Animal embryology, Stromal Cells metabolism
- Abstract
Mammary gland development is a complex process that is dependent on interactions between the developing mammary epithelium and the surrounding stromal tissues. We show that mice lacking the triglyceride synthesis enzyme acyl CoA:diacylglycerol transferase 1 (DGAT1) have impaired mammary gland development, characterized by decreased epithelial proliferation and alveolar development, and reduced expression of markers of functional differentiation. Transplantation studies demonstrate that the impaired development results from a deficiency of DGAT1 in both the stromal and epithelial tissues. Our findings are the first to link defects in stromal lipid metabolism to impaired mammary gland development.
- Published
- 2004
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