1. N6-Methyladenosine RNA Modification Regulates Shoot Stem Cell Fate in Arabidopsis
- Author
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Lu Liu, Hao Yu, Weiling Maggie Cai, Zhi Wei Norman Teo, Xingliang Hou, Zhe Liang, Ying Chen, Lisha Shen, Xiaofeng Gu, and Peter C. Dedon
- Subjects
0106 biological sciences ,0301 basic medicine ,Adenosine ,Meristem ,Arabidopsis ,RNA-binding protein ,01 natural sciences ,Article ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,Gene Expression Regulation, Plant ,RNA Processing, Post-Transcriptional ,Molecular Biology ,Genetics ,Messenger RNA ,biology ,Arabidopsis Proteins ,Methyltransferase complex ,Stem Cells ,MRNA modification ,fungi ,Gene Expression Regulation, Developmental ,RNA-Binding Proteins ,food and beverages ,RNA ,Cell Biology ,biology.organism_classification ,Cell biology ,Phenotype ,030104 developmental biology ,RNA, Plant ,Mutation ,Shoot ,Carrier Proteins ,Plant Shoots ,010606 plant biology & botany ,Developmental Biology - Abstract
N(6)-Methyladenosine (m(6)A) represents the most prevalent internal modification on mRNA and requires a multicomponent m(6)A methyltransferase complex in mammals. How their plant counterparts determine the global m(6)A modification landscape and its molecular link to plant development remain unknown. Here we show that FKBP12 INTERACTING PROTEIN 37 KD (FIP37) is a core component of the m(6)A methyltransferase complex, which underlies control of shoot stem cell fate in Arabidopsis. The mutants lacking FIP37 exhibit massive overproliferation of shoot meristems and a transcriptome-wide loss of m(6)A RNA modifications. We further demonstrate that FIP37 mediates m(6)A RNA modification on key shoot meristem genes inversely correlated with their mRNA stability, thus confining their transcript levels to prevent shoot meristem overproliferation. Our results suggest an indispensable role of FIP37 in mediating m(6)A mRNA modification, which is required for maintaining the shoot meristem as a renewable source for continuously producing all aerial organs in plants.
- Published
- 2016
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