1. Validation of an internal method for the diagnosis of infections with Chlamydophila abortus and Coxiella burnetii by real-time multiplex PCR.
- Author
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Pelletier C, Chartier S, Berthillier J, Dégletagne E, Rigaud C, Berthet H, Valognes A, Reynaud A, and Véry P
- Subjects
- Abortion, Veterinary microbiology, Animals, Cattle, Chlamydophila genetics, Chlamydophila Infections diagnosis, Chlamydophila Infections microbiology, Coxiella burnetii genetics, Polymerase Chain Reaction veterinary, Q Fever diagnosis, Q Fever microbiology, Reproducibility of Results, Sensitivity and Specificity, Cattle Diseases diagnosis, Cattle Diseases microbiology, Chlamydophila isolation & purification, Chlamydophila Infections veterinary, Coxiella burnetii isolation & purification, Polymerase Chain Reaction methods, Q Fever veterinary
- Abstract
Chlamydophila abortus and Coxiella burnetii are one of the major pathogens implicated in abortion in cattle. Their characteristic of obligate intracellular bacteria, and of zoonotic agents, makes their culture difficult in diagnostic laboratories, and the traditional tools of diagnosis (detection of sera antibodies by ELISA, Stamp's coloration) encounter specificity, sensitivity and interpretability limits. Individual PCR have recently been developed. Nevertheless, their income/cost is a limiting factor for breeders. As the symptoms are not specific, the request for analysis often concerns the two valences. Consequently, the development and the validation of an internal multiplex PCR appears to be a suitable solution.
- Published
- 2006