1. Passage of radio-iodinated insulin through the intestinal wall of the rat
- Author
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Muscogiuri A, D'Addabbo A, and Fanfani G
- Subjects
Electrophoresis ,Male ,Paper ,medicine.medical_specialty ,Endocrinology, Diabetes and Metabolism ,medicine.medical_treatment ,Protein-Losing Enteropathies ,Lumen (anatomy) ,Biology ,Iodine Radioisotopes ,Autoradiograph ,Aprotinin ,Intestinal mucosa ,Internal medicine ,Intestine, Small ,Internal Medicine ,medicine ,Animals ,Insulin ,Enteropathy ,Intestinal Mucosa ,Protein losing enteropathy ,Biological Transport ,medicine.disease ,Small intestine ,Rats ,Endocrinology ,medicine.anatomical_structure ,Injections, Intravenous ,Autoradiography ,Ion Exchange Resins ,medicine.drug - Abstract
131I-insulin was given intravenously to normal rats, and the passage of radioactivity into the duodenal loop containing a proteasis inhibitor was studied by two techniques. In the first, the 131I-insulin was separated from the intestinal content by electrophoresis; whereas in the second, the radioactivity that remained on an ion-exchange resin, directly introduced into the small intestine, was measured. It was found that within 15 minutes from the injection a small amount of 131I-insulin passes into the gut lumen. — The absorption of 131I-insulin from small intestine into the blood was studied after introduction of 131I-insulin along with a proteasis inhibitor into the intestinal loop. The autoradiograph of the electrophoresis of serum showed plasma-protein-bound radioactivity but no radioactivity due to unbound (free) insulin-131I. — These findings show that the passage of insulin through the intestinal wall of rats can be demonstrated by neutralizing the proteolytic activity in the intestinal tract. The possible role of this passage in protein-losing enteropathy is pointed out.
- Published
- 1967