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Your search keyword '"Fiorentino, Michelangelo"' showing total 9 results
Start Over Author "Fiorentino, Michelangelo" Journal diagnostic molecular pathology
9 results on '"Fiorentino, Michelangelo"'

4. Application of a Fluorescent PCR Method for Molecular Diagnosis of Posttransplant Lymphoproliferative Disorders on Routine Tissue Sections

Author

Gruppioni, Elisa, primary, Corti, Barbara, additional, Altimari, Annalisa, additional, Gabusi, Elena, additional, Panza, Emanuele, additional, Grazi, Gian Luca, additional, Pinna, Antonio Daniele, additional, Ruvo, Nicola De, additional, Fiorentino, Michelangelo, additional, Grigioni, Walter Franco, additional, and Grigioni, Antonia D??Errico, additional

Published
2005
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5. Genomic Allelotyping for Distinction of Recurrent and De Novo Hepatocellular Carcinoma After Orthotopic Liver Transplantation

Author

Altimari, Annalisa, primary, Gruppioni, Elisa, additional, Fiorentino, Michelangelo, additional, Petraroli, Rosella, additional, Pinna, Antonio Daniele, additional, Petropulacos, Kyriakoula, additional, Ridolfi, Lorenza, additional, Costa, Alessandro Nanni, additional, Grigioni, Walter Franco, additional, and Grigioni, Antonia D??Errico, additional

Published
2005
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9. High Levels of BCL2 Messenger RNA Detected by In Situ Hybridization in Human Hepatocellular and Cholangiocellular Carcinomas

Author

Fiorentino, Michelangelo, D'Errico, Antonia, Altimari, Annalisa, Barozzi, Chiara, and Grigioni, Walter F.

Abstract

Immunocytochemistry has indicated that, in the liver, the bcl-2 gene is generally expressed in bile duct cells and tumors of biliary origin. Both in situ hybridization and immunocytochemistry were used to analyze the expression of bcl-2 messenger RNA (mRNA) and its protein product (Bcl-2) in the tissue of 50 pure primary liver tumor (PLT) specimens including 40 hepatocellular carcinoma (HCC) specimens and 10 cholangiocellular carcinoma (CC) specimens. The phenotype of the tumors expressing bcl-2 was confirmed by immunocytochemical assessment of the cytokeratin (CK) profile (CK8, CK18, CK7, and CK19). Whereas positive immunoreaction with the anti-Bcl-2 MoAb was revealed in only 8 (20) of 40 HCC specimens and 1 (10) of 10 CC specimens, high contents of bcl-2 mRNA were found in 26 (65) of 40 HCC specimens and 9 (90) of 10 CC specimens. Regarding the CK profile, only 25 (62) of 40 HCC specimens showed pure hepatocytic lineage (CKs 8–18), whereas among the remaining 15 HCC specimens, positivity for either CK7 (12 specimens) or CK19 (5 specimens) was observed. All 10 CC specimens stained with CKs 8–18–19, and 8 of 10 stained with CK 7 as well. These results indicate that PLTs display a greater expression of bcl-2 mRNA than of the Bcl-2 protein. Furthermore, CK profile assessment confirmed that bcl-2 expression is not confined to liver tumors of biliary origin. In the absence of a well-demonstrated post-transcriptional control of the gene, the authors propose the detection of bcl-2 mRNA by in situ hybridization as a possible alternative method for assessing the expression of bcl-2 mRNA in PLT.

Published
1999

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