1. One-step detection and genotyping of human papillomavirus in cervical samples by reverse hybridization
- Author
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Sergio Laconi, Pietro Pellegrini-Bettoli, Marco Rais, Paolo Pani, Marianna Greco, and Ezio Laconi
- Subjects
Serial dilution ,Genotype ,Uterine Cervical Neoplasms ,Biology ,Cervical intraepithelial neoplasia ,Polymerase Chain Reaction ,Sensitivity and Specificity ,Pathology and Forensic Medicine ,Consensus Sequence ,medicine ,Humans ,Molecular Biology ,Genotyping ,Papillomaviridae ,Polymerase ,DNA Primers ,Nucleic Acid Hybridization ,Histology ,Cell Biology ,Assay sensitivity ,Amplicon ,medicine.disease ,Uterine Cervical Dysplasia ,Molecular biology ,female genital diseases and pregnancy complications ,DNA, Viral ,biology.protein ,Female ,Restriction fragment length polymorphism ,Polymorphism, Restriction Fragment Length - Abstract
This study describes a nonisotopic polymerase chain reaction-reverse hybridization-based method (PCR-RH) for the one-step detection and genotyping of anogenital human papillomavirus (HPV) in a microwell format. HPV DNA was amplified and labeled by PCR using GP5+/GP6+ primers. Labeled amplicons were hybridized to 20 HPV type-specific capture probes anchored to the surface of plastic microwells and detected by an immunoenzymatic assay. Assay sensitivity was
- Published
- 2001