1. Mechanisms of epithelial thickening due to IL-1 signalling blockade and TNF-α administration differ during wound repair and regeneration.
- Author
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Abarca-Buis RF, Martínez-Jiménez A, Vera-Gómez E, Contreras-Figueroa ME, Garciadiego-Cázares D, Paus R, Robles-Tenorio A, and Krötzsch E
- Subjects
- Animals, Disease Models, Animal, Mice, Inbred C57BL, Regeneration drug effects, Cicatrix drug therapy, Interleukin 1 Receptor Antagonist Protein drug effects, Tumor Necrosis Factor-alpha pharmacology, Wound Healing drug effects
- Abstract
IL-1 and TNF-α are always present during wound repair, but their pleiotropic and synergistic effects are incompletely understood. In this work, we evaluated the role of IL-1 in wound repair, and examined whether TNF-α administration impaired scarless wound repair. First, we characterised wound repair in outbred CD-1 mice according to age and sex in an ear punch wound model. Then, we examined the effects of Interleukin 1 receptor antagonist (IL-1ra) and TNF-α placement inside ear wounds by means of loaded Heparin beads in young and middle-aged male and female mice. Wounds in middle-aged females repaired with scarless characteristics, whereas those in young males showed fibrotic scarring. Rather than improving wound repair in young males, IL-1 signalling blockade increased epithelial thickness and IL-1β and TNF-α expression, and diminished epidermal apoptosis. TNF-α impaired wound repair in middle-aged females, which exhibited acanthosis and overexpression of IL-1, but no change in apoptosis. These findings suggest that this mechanism of epidermal thickening differs from that observed in IL1-ra-treated animals., (Copyright © 2017 International Society of Differentiation. Published by Elsevier B.V. All rights reserved.)
- Published
- 2018
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